Biogas obtainment and other compounds of biotechnological interest from citrus/orange processing residues

• Grant number: 17/01722-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: June 01, 2017
• End date: February 28, 2021
• Field of knowledge: Engineering - Sanitary Engineering - Environmental Sanitation
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Maria Bernadete Amâncio Varesche
• Grantee: Franciele Pereira Camargo
• Host Institution: Escola de Engenharia de São Carlos (EESC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Associated research grant: 15/06246-7 - Biorefinery concept applied to biological wastewater treatment plants: environmental pollution control coupled with material and energy recovery, AP.TEM
• Associated scholarship(s): 18/26182-1 - Microbial characterization of a consortium involved in an optimized batch reactor applied to biohydrogen production from citrus peel waste as lignocellulosic substrate, BE.EP.DR

Abstract

Brazil is considered the main producer of citrus/orange and it is estimated that the residues generated during the processing of this raw material can reach up to 60% of the total produced, being composed mainly by the fruit peels and bagasse. One of the obstacles to the production of compounds of biotechnological interest, such as methane, hydrogen, organic acids and alcohols, from these residues is the difficult degradation of some of its components, such as lignin, cellulose and hemicellulose. Thus, it is necessary to apply physical, chemical or biological pretreatment for promoting hydrolysis of lignocellulosic components. This research aims to evaluate the obtainment of compounds of biotechnological interest from citrus/orange processing residues using thermophilic UASB sludge applied to the treatment of vinasse sugarcane as inoculum. The experiments will be performed in batch-operated flasks using different strategies for breaking the lignocellulosic structure of the orange residues. For this purpose, the statistical method of Experimental Planning will be used by means of the response surface to determine the best variables of the experimental conditions (temperature, concentration of yeast extract and pH) in relation to the strategy of rupture of the lignocellulosic structure (hydrothermolysis or alkaline hydrolysis with hydrogen peroxide) and also of the substrate used (peels, bagasse or peels + bagasse together). The microbial community will be evaluated using the large-scale sequencing technique of the 16S RNAr gene via the Illumina. (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CAMARGO, FRANCIELE PEREIRA; SALZAMOTO, ISABEL KIMIKO; SILVEIRA DUARTE, IOLANDA CRISTINA; AMANCIO VARESCHE, MARIA BERNADETE. Influence of alkaline peroxide assisted and hydrothermal pretreatment on biodegradability and bio-hydrogen formation from citrus peel waste. INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, v. 44, n. 41, p. 22888-22903, AUG 30 2019. (17/01722-0, 15/06246-7)

CAMARGO, FRANCIELE PEREIRA; SAKAMOTO, ISABEL KIMIKO; DELFORNO, TIAGO PALLADINO; MARIADASSOU, MAHENDRA; LOUX, VALENTIN; MIDOUX, CEDRIC; SILVEIRA DUARTE, IOLANDA CRISTINA; SILVA, EDSON LUIZ; BIZE, ARIANE; AMANCIO VARESCHE, MARIA BERNADETE. Microbial and functional characterization of an allochthonous consortium applied to hydrogen production from Citrus Peel Waste in batch reactor in optimized conditions. Journal of Environmental Management, v. 291, AUG 1 2021. (17/01722-0, 15/06246-7, 18/26182-1)

CAMARGO, FRANCIELE PEREIRA; SAKAMOTO, ISABEL KIMIKO; SILVA, EDSON LUIZ; DUARTE, IOLANDA CRISTINA SILVEIRA; VARESCHE, MARIA BERNADETE AMANCIO. Bioaugmentation with Enterococcus casseliflavus: A Hydrogen-Producing Strain Isolated from Citrus Peel Waste. WASTE AND BIOMASS VALORIZATION, v. 12, n. 2, SI, p. 895-911, FEB 2021. (15/06246-7, 17/01722-0)

FRANCIELE PEREIRA CAMARGO; ARNALDO SARTI; ALBERTO CAMILO ALÉCIO; CAROLINA APARECIDA SABATINI; MARIA ANGELA TALLARICO ADORNO; IOLANDA CRISTINA SILVEIRA DUARTE; MARIA BERNADETE AMÂNCIO VARESCHE. LIMONENE QUANTIFICATION BY GAS CHROMATOGRAPHY WITH MASS SPECTROMETRY (GC-MS) AND ITS EFFECTS ON HYDROGEN AND VOLATILE FATTY ACIDS PRODUCTION IN ANAEROBIC REACTORS. Química Nova, v. 43, n. 7, p. 844-850, 2020-08-21. (17/01722-0, 15/15880-1)

CAMARGO, FRANCIELE PEREIRA; SAKAMOTO, ISABEL KIMIKO; DELFORNO, TIAGO PALLADINO; MIDOUX, CEDRIC; SILVEIRA DUARTE, IOLANDA CRISTINA; SILVA, EDSON LUIZ; BIZE, ARIANE; AMANCIO VARESCHE, MARIA BERNADETE. Microbial and functional characterization of granulated sludge from full-scale UASB thermophilic reactor applied to sugarcane vinasse treatment. ENVIRONMENTAL TECHNOLOGY, v. N/A, p. 20-pg., 2022-04-06. (15/06246-7, 18/26182-1, 17/01722-0)

CAMARGO, FRANCIELE PEREIRA; SAKAMOTO, ISABEL KIMIKO; BIZE, ARIANE; DUARTE, IOLANDA CRISTINA SILVEIRA; SILVA, EDSON LUIZ; VARESCHE, MARIA BERNADETE AMANCIO. Screening design of nutritional and physicochemical parameters on bio-hydrogen and volatile fatty acids production from Citrus Peel Waste in batch reactors. INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, v. 46, n. 11, p. 7794-7809, FEB 11 2021. (17/01722-0, 18/26182-1, 15/06246-7)

CAMARGO, FRANCIELE P.; RABELO, CAMILA A. B. S.; DUARTE, IOLANDA C. S.; SILVA, EDSON L.; VARESCHE, MARIA BERNADETE A.. Biogas from lignocellulosic feedstock: A review on the main pretreatments, inocula and operational variables involved in anaerobic reactor efficiency. INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, v. 48, n. 54, p. 20-pg., 2023-06-07. (17/01722-0, 15/06246-7)

CAMARGO, FRANCIELE PEREIRA; SAKAMOTO, ISABEL KIMIKO; DUARTE, IOLANDA CRISTINA SILVEIRA; SILVA, EDSON LUIZ; VARESCHE, MARIA BERNADETE AMANCIO. Metataxonomic characterization of bacterial and archaeal community involved in hydrogen and methane production from citrus peel waste (Citrus sinensis L. Osbeck) in batch reactors. BIOMASS & BIOENERGY, v. 149, JUN 2021. (17/01722-0, 15/06246-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CAMARGO, Franciele Pereira. Taxonomic and functional characterization of the microbial community involved in obtaining biogas and other compounds of biotechnological interest from citrus peel waste. 2021. Doctoral Thesis - Universidade de São Paulo (USP). Escola de Engenharia de São Carlos (EESC/SBD) São Carlos.