Screening design of nutritional and physicochemical parameters on bio-hydrogen and volatile fatty acids production from Citrus Peel Waste in batch reactors

Author(s)

Camargo, Franciele Pereira ^[1] ; Sakamoto, Isabel Kimiko ^[1] ; Bize, Ariane ^[2] ; Duarte, Iolanda Cristina Silveira ^[3] ; Silva, Edson Luiz ^[4] ; Varesche, Maria Bernadete Amancio ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Engn Sao Carlos, Dept Hydraul & Sanitat, Av Trabalhador Sao Carlense 400, BR-13566590 Sao Carlos, SP - Brazil

^[2] Univ Paris Saclay, INRAE, PROSE, 1 Rue Pierre Gilles de Gennes, CS10030, F-92761 Antony - France

^[3] Fed Univ Sao Carlos UFSCar, Dept Biol, Km 101, BR-18052780 Sorocaba, SP - Brazil

^[4] Univ Fed Sao Carlos, Dept Chem Engn, Rod Washington Luiz, Km 235, SP 310, BR-13565905 Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF HYDROGEN ENERGY; v. 46, n. 11, p. 7794-7809, FEB 11 2021.
• Web of Science Citations: 0

Abstract

Different nutritional and physicochemical conditions to obtain H2 from Citrus Peel Waste (CPW) were evaluated. For this, a screening design was carried out using Plackett and Burman design, in order to verify the main significant conditions of this process. The variables studied were pH (5.5-8.5), temperature (30-44 degrees C), autochthonous inoculum (0.75-2.25 gTVS.L-1), allochthonous inoculum (1-3 gTVS.L-1) and substrate (5-15 g.L-1), headspace (40-60%) and nutritional medium components, such as yeast extract (0-1 g.L-1), CaCO3, NaCl and peptone (0-5 g.L-1). The most significant operational variables were pH (8.5), allochthonous inoculum (3 gTVS.L-1) and substrate concentration (15 g CPW.L-1), conditions that favored the highest H-2 (13.29 mmol.L-1) and acetic acid productions (1340 mg.L-1). Escherichia (34.5%) and Clostridium (29.93%) were the main genera identified under these conditions. (c) 2020 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 17/01722-0 - Biogas obtainment and other compounds of biotechnological interest from citrus/orange processing residues
• Grantee: Franciele Pereira Camargo
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 18/26182-1 - Microbial characterization of a consortium involved in an optimized batch reactor applied to biohydrogen production from citrus peel waste as lignocellulosic substrate
• Grantee: Franciele Pereira Camargo
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 15/06246-7 - Biorefinery concept applied to biological wastewater treatment plants: environmental pollution control coupled with material and energy recovery
• Grantee: Marcelo Zaiat
• Support Opportunities: Research Projects - Thematic Grants