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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The effect of nanoscale surface electrical properties of partially biodegradable PEDOT-co-PDLLA conducting polymers on protein adhesion investigated by atomic force microscopy

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Author(s):
da Silva, Arua C. [1] ; Higgins, Michael J. [2] ; Cordoba de Torresi, Susana I. [1]
Total Authors: 3
Affiliation:
[1] Univ Sao Paulo, Inst Quim, Ave Prof Lineu Prestes 748, BR-05508000 Sao Paulo, SP - Brazil
[2] Univ Wollongong, Intelligent Polymer Res Inst, ARC Ctr Excellence Electromat Sci, Wollongong, NSW 2522 - Australia
Total Affiliations: 2
Document type: Journal article
Source: Materials Science & Engineering C-Materials for Biological Applications; v. 99, p. 468-478, JUN 2019.
Web of Science Citations: 2
Abstract

This study used atomic force microscopy (AFM) to elucidate the interaction of fibronectin (FN) on a conducting and partially biodegradable copolymer of poly(3,4-ethylenedioxythiophene) and poly(D,L-lactic acid) (PEDOT-co-PDLLA) in three different proportions (1:05, 1:25 and 1:50). The copolymers with higher PEDOT:PDLLA content ratios (1:05 and 1:25) had higher surface roughness, water contact angle, with current and conductivity occurring at discrete large grain structures on the surface. In contrast, the lower PEDOT:PDLLA content ratio (1:50) did not show high conductivity grains but showed homogenous surface conductivity across the entire surface. Using FN-functionalized AFM probes, force measurements showed that the copolymers with higher PEDOT content (1:05 and 1:25) had significantly lower adhesion forces (similar to 0.2-0.3 nN), while the copolymer with the lower content of PEDOT (1:50) had stronger FN interactions with significantly higher adhesion forces of 1 nN. By correlating the spatially distributed electrical surfaces with FN interactions, we observed that the synthesis of 1:50 PEDOT:PDLLA produced more uniformly doped polymer films that facilitated FN adsorption through favorable interactions with accessible sulfate dopants. Importantly, these findings are correlated with previous studies showing increased stem cell migration and differentiation on 1:50 PEDOT:PDLLA surfaces compared with surfaces with 1:05 and 1:25 PEDOT:PDLLA ratios. (AU)

FAPESP's process: 14/09353-6 - Biomaterials based on conducting biodegradable and biocompatible polymers
Grantee:Aruã Clayton da Silva
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 12/50880-4 - Stem cells: from basic studies of kinin and purinergic receptor roles towards therapeutical applications
Grantee:Alexander Henning Ulrich
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 17/00705-5 - Study of interaction between different proteins with a PEDOT-co-PLLA surface by EC-AFM
Grantee:Aruã Clayton da Silva
Support Opportunities: Scholarships abroad - Research Internship - Doctorate (Direct)
FAPESP's process: 15/26308-7 - Optimization of the physicochemical properties of nano -structured materials for applications in molecular recognition, catalysis and energy conversion/storage
Grantee:Roberto Manuel Torresi
Support Opportunities: Research Projects - Thematic Grants