| Full text | |
| Author(s): |
Thompson, Francis Cazzeli
[1]
;
Matsumoto, Mariza Akemi
[2]
;
Biguetti, Claudia Cristina
[3]
;
Muniz Renno, Ana Claudia
[4]
;
Holgado, Leandro de Andrade
[1]
;
Santiago Junior, Joel Ferreira
[1]
;
Munerato, Marcelo Salles
[1]
;
Saraiva, Patricia Pinto
[3]
Total Authors: 8
|
| Affiliation: | [1] Sagrado Coracao Univ USC, Oral Biol Postgrad Program, Rua Irma Arminda 10-50, BR-17011160 Bauru, SP - Brazil
[2] Sao Paulo State Univ UNESP, Sch Dent, Dept Basic Sci, Rua Jose Bonifacio 1193, BR-16015050 Aracatuba, SP - Brazil
[3] Univ Oeste Paulista UNOESTE, Fac Med Jau, R Angelo Martins 498, BR-17203480 Jau, SP - Brazil
[4] Univ Fed Sao Paulo, Dept Biosci, Campus Baixada Santista UNIFESP, Ave Ana Costa, 95, BR-11060001 Santos, SP - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Materials Science & Engineering C-Materials for Biological Applications; v. 99, p. 726-734, JUN 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
Objectives: To follow healing process of augmented maxillary sinus in rabbits analyzing the histological pattern of bone tissue formation, along with the osteogenic activity and vascularization using a bioactive vitroceramic in comparison to deproteinized bovine bone associated or not with autogenous bone graft. Design: Forty five male adult New Zealand rabbits, 5 months of age, mean weight of 4 Kg, underwent bilateral sinus augmentation surgeries to be divided in five groups: G - (Control) particulate autogenous bone graft (AG), BO - deproteinized bovine bone, BO+G - deproteinized bovine bone + AG, BSi - vitroceramic, and BSi + G - vitroceramic + AG. After 15, 45 and 90 days, all animals were euthanized for specimen's removal to be analyzed under light microscopy, histomorphometry, and immunohistochemistry for Runx2 and VEGF labeling. Results: G, BO and BO + G groups healed uneventfully, allowing the formation of mature remodeling bone at day 90, regarding the association of AG with the biomaterial. On the other hand, BSi and BSi + G groups showed an important cellular reaction and granulation/fibrous tissue formation from the first to the last period of observation. Runx-2 and VEGF immunolabeling were coherent with this result. However, histomorphometry did not reveal significant differences considering new bone formation. Conclusions: Reconstructed maxillary sinuses using Biosilicate (R) permitted satisfactory new bone formation in comparison to the deproteinized bovine bone and AG. However, the presence of granulation/fibrous tissue and inflammatory cells associated to the degrading biomaterial indicate that further studies should be careful performed considering the immunological aspect of this new biomaterial. (AU) | |
| FAPESP's process: | 16/03762-7 - Macrophages phenotype identification during repair of bone defect's reconstructed with different biomaterials and their influence on healing process and on the quality of newly formed bone |
| Grantee: | Mariza Akemi Matsumoto |
| Support Opportunities: | Regular Research Grants |