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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Adipogenic differentiation of murine bone marrow mesenchymal stem cells induced by visible light via photo- induced biomodulation

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Author(s):
Castilho-Fernandes, Andrielle [1] ; Lopes, Tadla G. [1] ; Ferreira, Fernando U. [2, 3] ; Rezende, Nayara [1] ; Silva, Valeria F. [1, 2, 3] ; Primo, Fernando L. [1, 4] ; Fontes, Aparecida Maria [5] ; Ribeiro-Silva, Alfredo [6] ; Tedesco, Antonio Claudio [1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Ctr Nanotechnol & Tissue Engn, Dept Chem, Photobiol & Photomed Res Grp, BR-14040901 Ribeirao Preto - Brazil
[2] Univ Sao Paulo, Ctr Cell Therapy, Med Sch, Natl Inst Sci & Technol Stem Cell & Cell Therapy, BR-14051140 Ribeirao Preto - Brazil
[3] Univ Sao Paulo, Reg Blood Ctr, Med Sch, Natl Inst Sci & Technol Stem Cell & Cell Therapy, BR-14051140 Ribeirao Preto - Brazil
[4] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Bioproc & Biotechnol, Araraquara SP 14800-903, BR-14800903 Araraquara, SP - Brazil
[5] Univ Sao Paulo, Med Sch, Dept Genet, BR-14051140 Ribeirao Preto - Brazil
[6] Univ Sao Paulo, Med Sch, Dept Pathol, BR-14049900 Ribeirao Preto - Brazil
Total Affiliations: 6
Document type: Journal article
Source: Photodiagnosis and Photodynamic Therapy; v. 25, p. 119-127, MAR 2019.
Web of Science Citations: 1
Abstract

Background: Bone marrow mesenchymal stem cells (BM-MSCs) are undifferentiated cells that can proliferate and differentiate into specialized cells for tissue self-repair. Low-level laser (LLL) can induce biomodulatory effects such as cellular proliferation, differentiation, and migration. We investigated the biomodulatory effects of the photoactive compound chloroaluminum phthalocyanine nanoemulsion (AlClPc/NE) on the adipogenic differentiation of BM-MSCs, when combined with LLL (AlClPc/NE-LLL). Methods: The BM-MSCs used in this work were isolated from green fluorescent protein-positive (GFP(+)) C57BL6 mice. Cells were first treated with AlClPc/NE, a well-designed photoactive nano-drug and were then subjected to in vitro expansion, morphological and immunophenotypic characterization, and cellular cytotoxicity analysis. Subsequently, BM-MSCs were induced to differentiate into adipocytes by photo-induced biomodulation with AlClPc/NE-LLL. Results: Our results showed that the isolated cell population was consistent with murine BM-MSCs. The cellular cytotoxicity analysis revealed that the optimal nanoemulsion dose to induce BM-MSC biomodulation was 5.0 mu mol/L. Twenty-four hours following treatment with AlClPc/NE, BM-MSC were subjected to visible light irradiation of 20 mJ/cm(2) at 670 nm. Six days after photo-induced biomodulation, cells maintained high GFP expression level, and expressed detectable mRNA levels of adipogenic genes (lipoprotein lipase and PPAR gamma); formation of lipid vacuoles was observed, and the cells did not show any tumorigenic potential in vivo. Conclusions: Our results indicated that photo-induced biomodulation via visible light using AlClPc/NE and LLL can induce adipogenic differentiation of murine BM-MSCs. Therefore, cell therapy with BM-MSCs and photoinduced biomodulation may contribute to the development of new therapeutic strategies that are faster and more effective than traditional methods to trigger MSC differentiation. (AU)

FAPESP's process: 13/50181-1 - Use of drugs containing nanocarriers with photosensitizers and/or other active compounds applied to cell therapy and treatment of central nervous system disorders
Grantee:Antonio Claudio Tedesco
Support type: Research Projects - Thematic Grants