An Innovative Biocatalyst for Continuous 2G Ethanol Production from Xylo-Oligomers by Saccharomyces cerevisiae through Simultaneous Hydrolysis, Isomerization, and Fermentation (SHIF)

Author(s)

Milessi-Esteves, Thais S. ^[1] ; Corradini, Felipe A. S. ^[2] ; Kopp, Willian ^[1] ; Zangirolami, Teresa C. ^{[2, 1]} ; Tardioli, Paulo W. ^{[2, 1]} ; Giordano, Roberto C. ^{[2, 1]} ; Giordano, Raquel L. C. ^{[2, 1]}

Affiliation

^[1] Univ Fed Sao Carlos, Dept Chem Engn, Rodovia Washington Luiz, Km 235, BR-13565905 Sao Carlos, SP - Brazil

^[2] Fed Univ Sao Carlos PPGEQ UFSCar, Grad Program Chem Engn, Rodovia Washington Luiz, Km 235, BR-13565905 Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: CATALYSTS; v. 9, n. 3 MAR 2019.
• Web of Science Citations: 0

Abstract

Many approaches have been considered aimed at ethanol production from the hemicellulosic fraction of biomass. However, the industrial implementation of this process has been hindered by some bottlenecks, one of the most important being the ease of contamination of the bioreactor by bacteria that metabolize xylose. This work focuses on overcoming this problem through the fermentation of xylulose (the xylose isomer) by native Saccharomyces cerevisiae using xylo-oligomers as substrate. A new concept of biocatalyst is proposed, containing xylanases and xylose isomerase (XI) covalently immobilized on chitosan, and co-encapsulated with industrial baker's yeast in Ca-alginate gel spherical particles. Xylo-oligomers are hydrolyzed, xylose is isomerized, and finally xylulose is fermented to ethanol, all taking place simultaneously, in a process called simultaneous hydrolysis, isomerization, and fermentation (SHIF). Among several tested xylanases, Multifect CX XL A03139 was selected to compose the biocatalyst bead. Influences of pH, Ca2+, and Mg2+ concentrations on the isomerization step were assessed. Experiments of SHIF using birchwood xylan resulted in an ethanol yield of 0.39 g/g, (76% of the theoretical), selectivity of 3.12 g(ethanol)/g(xylitol), and ethanol productivity of 0.26 g/L/h. (AU)

• FAPESP's process: 16/10636-8 - From the cell factory to the Biodiesel-Bioethanol integrated biorefinery: a systems approach applied to complex problems in micro and macroscales
• Grantee: Roberto de Campos Giordano
• Support type: Program for Research on Bioenergy (BIOEN) - Thematic Grants
• FAPESP's process: 08/56246-0 - Bioprocess Systems Engineering (BSE) applied to the production of bioethanol from sugarcane bagasse
• Grantee: Roberto de Campos Giordano
• Support type: Program for Research on Bioenergy (BIOEN) - Thematic Grants