Resveratrol as a Therapy to Restore Neurogliogenesis of Neural Progenitor Cells Infected by Toxoplasma gondii

Author(s):Show less -

Bottari, Nathieli B. ^{[1, 2]} ; Schetinger, Maria Rosa C. ^{[1, 2]} ; Pillat, Micheli M. ^[3] ; Palma, Thais V. ^{[1, 2]} ; Ulrich, Henning ^[3] ; Alves, Mariana S. ^{[1, 2]} ; Morsch, Vera M. ^{[1, 2]} ; Melazzo, Cinthia ^{[1, 2]} ; de Barros, Luiz Daniel ^[4] ; Garcia, Joao Luis ^[4] ; Da Silva, Aleksandro Schafer ^{[1, 2, 5]}

Affiliation

^[1] Univ Fed Santa Maria, Grad Program Toxicol Biochem, Ave Roraima 1000, BR-97105900 Santa Maria, RS - Brazil

^[2] Univ Fed Santa Maria, Dept Biochem & Mol Biol, Ave Roraima 1000, BR-97105900 Santa Maria, RS - Brazil

^[3] Univ Sao Paulo, Inst Chem, Dept Biochem, Sao Paulo, SP - Brazil

^[4] Univ Estadual Londrina, Dept Vet Prevent Med, Londrina, Parana - Brazil

^[5] Univ Estado Santa Catarina UDESC, Grad Program Anim Sci, Chapeco, SC - Brazil

• Document type: Journal article
• Source: Molecular Neurobiology; v. 56, n. 4, p. 2328-2338, APR 2019.
• Web of Science Citations: 3

Abstract

The intracellular protozoan Toxoplasma gondii may cause congenital toxoplasmosis and serious brain damage in fetus. However, the underlying mechanism of neuropathogenesis in brain toxoplasmosis remains unclear. For this study, neural progenitor cells (NPCs) were obtained from embryo telencephalons (embryonic day 13) and induced to proliferation in the presence of growth factors (GFs). For gathering insights into the biological effects of resveratrol (RSV) on neurogenesis, this study aimed to investigate effects of RSV concentrations (0.1 to 100M) on proliferation, migration and differentiation of NPCs infected by T. gondii. T. gondii infection increased the presence of cells in Sub G1 phase, reducing the global frequency of undifferentiated cells in S and G2/M phases of cell cycle and reduced cell viability/mithochondrial activity of infected NPCs. Moreover T. gondii stimulated neural migration and gliogenesis during neutral differentation. However, the treatment with RSV stimulated cell proliferation, restored cellular viability of infected NPCs and exerted an inhibitory effect on gliogenesis of infected NPCs favorecing neuronal maturation during toxoplasmosis infection. Thus, we have successfully to demonstrated that RSV is promising as therapeutic for congenital toxoplasmosis. (AU)

• FAPESP's process: 12/50880-4 - Stem cells: from basic studies of kinin and purinergic receptor roles towards therapeutical applications
• Grantee: Alexander Henning Ulrich
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 15/19478-3 - Role of kinin-B2 receptor in pathogenesis and progression of familial Alzheimer's Disease: from neurogenesis and immune response to cognition
• Grantee: Micheli Mainardi Pillat
• Support Opportunities: Scholarships in Brazil - Post-Doctoral