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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The Dynamics of Replication in Trypanosoma cruzi Parasites by Single-Molecule Analysis

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de Araujo, Christiane B. [1, 2] ; Calderano, Simone G. [3, 2] ; Elias, Maria Carolina [1, 2]
Total Authors: 3
[1] Inst Butantan, Lab Especial Ciclo Celular, Ave Vital Brasil 1500, BR-05503900 Sao Paulo - Brazil
[2] Inst Butantan, Ctr Toxins Immune Response & Cell Signaling CeTIC, Ave Vital Brasil 1500, BR-05503900 Sao Paulo - Brazil
[3] Inst Butantan, Lab Parasitol, Ave Vital Brasil 1500, BR-05503900 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Journal of Eukaryotic Microbiology; v. 66, n. 3, p. 514-518, MAY-JUN 2019.
Web of Science Citations: 0

Here, we investigated the features of replication in Trypanosoma cruzi epimastigotes based on fork speed progression, which is influenced by distinct features such as DNA polymerase rate, susceptibility to DNA damage and repair, secondary structures, transcription and chromatin state. Although T. cruzi exhibits a mean fork speed (2.05 +/- 0.10 kb/min) very similar to other trypanosomatids, we found that the majority of DNA molecules replicated more slowly, with a frequency distribution approximately 1 kb/min. This frequency distribution analysis provides more information about the replication profile of this organism. (AU)

FAPESP's process: 13/07467-1 - CeTICS - Center of Toxins, Immune-Response and Cell Signaling
Grantee:Hugo Aguirre Armelin
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 16/50050-2 - How do common and diverged features of the replicative stress response shape the biology of TriTryp parasites?
Grantee:Maria Carolina Quartim Barbosa Elias Sabbaga
Support type: Research Projects - Thematic Grants
FAPESP's process: 14/13375-5 - Replication origins in trypanosomes
Grantee:Christiane Bezerra de Araujo
Support type: Scholarships in Brazil - Post-Doctorate