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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Identification of Hfq-binding RNAs in Caulobacter crescentus

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Assis, Nadine G. [1] ; Ribeiro, Rodolfo A. [1] ; da Silva, Larissa G. [1] ; Vicente, Alexandre M. [1] ; Hug, Isabelle [2] ; Marques, Marilis V. [1]
Total Authors: 6
[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, Sao Paulo - Brazil
[2] Univ Basel, Biozentrum, Basel - Switzerland
Total Affiliations: 2
Document type: Journal article
Source: RNA BIOLOGY; v. 16, n. 6 MAR 2019.
Web of Science Citations: 0

Small RNAs are important for post-transcriptional regulation of gene expression, affecting stability and activity of their target mRNAs. The bacterial Sm-like protein Hfq is required to promote pairing between both RNAs when their sequence complementarity is limited. To provide a first global view on the post-transcriptional landscape of the alpha-proteobacterium Caulobacter crescentus, we have identified the Hfq-binding RNAs employing High-throughput sequencing of RNA isolated by cross-linking immunoprecipitation (HITS-CLIP). A total of 261 RNAs, including 3 unannotated RNAs, were successfully identified and classified according to putative function. Moreover, possible interactions between the identified sRNAs with mRNA targets were postulated through computational target predictions. (AU)

FAPESP's process: 16/06378-3 - Characterization of the DEAD-box RNA helicase CC1478 in Caulobacter crescentus and its regulation
Grantee:Rodolfo Alvarenga Ribeiro
Support type: Scholarships in Brazil - Master
FAPESP's process: 14/13552-4 - Study of the DEAD-box RNA helicase encoded by the gene CC0835 in Caulobacter crescentus
Grantee:Alexandre Magno Vicente
Support type: Scholarships in Brazil - Master
FAPESP's process: 14/04046-8 - Regulatory networks of bacterial stress response
Grantee:Marilis Do Valle Marques
Support type: Research Projects - Thematic Grants
FAPESP's process: 15/21025-7 - Hfq protein heterologous expression and FLAG-Hfq line obtention with induced expression
Grantee:Nadine Gimenez de Assis
Support type: Scholarships in Brazil - Scientific Initiation