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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

One-Pot Synthesis and Antifungal Activity of Nontoxic Silver-Loaded Hydroxyapatite Nanocomposites against Candida Species

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Gottardo, Bianca [1] ; Lemes, Thiago H. [1] ; Byzynski, Gabriela [2] ; Paziani, Mario H. [3] ; von-Zeska-Kress, Marcia R. [3] ; de Almeida, Margarete T. G. [2] ; Volanti, Diogo P. [1]
Total Authors: 7
[1] Sao Paulo State Univ UNESP, Inst Biosci Humanities & Exact Sci, R Cristovao Colombo 2265, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil
[2] Sao Jose do Rio Preto Med Sch FAMERP, Ave Brigadeiro Faria Lima 5416, BR-15090000 Sao Jose Do Rio Preto, SP - Brazil
[3] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Ave Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: ACS APPLIED NANO MATERIALS; v. 2, n. 4, p. 2112-2120, APR 2019.
Web of Science Citations: 1

The impact of fungal diseases and the development of antimicrobial agents against pathogenic fungi have emerged as a main global healthcare challenge. In this study, the antifungal activity of silver-loaded hydroxyapatite (Ag/HAP) nanocomposites (NCs) with different Ag content synthesized by a one-pot microwave assisted solvothermal method was evaluated against sensitive and resistant Candida species. The NCs' composition and morphology were characterized by X-ray diffraction, field emission scanning electron microscopy, energy-dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy, and high-resolution transmission electron microscopy analysis. Antifungal studies were conducted by a microdilution method according to a protocol from the Clinical and Laboratory Standards Institute. The main inhibitory effect was observed against Candida krusei, with a minimum inhibitory concentration (MIC) of 31.2 mu g/mL, followed by Candida parapsilosis sensu stricto and Candida tropicalis (62.5 mu g/mL) and Candida glabrata and Candida albicans (125 mu g/mL). Furthermore, a toxicity assay was performed in the in vivo model Galleria mellonella by bathing or inoculating with the same NC concentration used in the previously mentioned microdilution experiments. For both approaches, all NC concentrations were not toxic in the in vivo model. The specific antifungal activity demonstrated that NCs act efficiently against species of Candida. These results show a potential antifungal application for well-designed nanostructured Ag/HAP composites. (AU)

FAPESP's process: 15/04511-5 - Photocatalytic activity evaluation and degradation mechanism of doped semiconductor and graphene heterostructure
Grantee:Gabriela Byzynski Soares
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 17/01267-1 - Graphene acid-hollow metal oxides composites for gas sensor
Grantee:Diogo Paschoalini Volanti
Support type: Regular Research Grants