Genetic Structure of Brazilian Populations of Triatoma sordida (Stal, 1859) (Hemiptera, Triatominae) by Means of Chromosomal Markers

Author(s)

Madeira, Fernanda Fernandez ^[1] ; dos Reis, Yago Visinho ^[1] ; Bittinelli, Isadora de Freitas ^{[1, 2]} ; Grzyb Delgado, Luiza Maria ^[1] ; de Oliveira, Jader ^[2] ; Mendonca, Vagner Jose ^[3] ; Figueiredo Moreira, Felipe Ferraz ^[4] ; Vilela de Azeredo-Oliveira, Maria Tercilia ^[1] ; da Rosa, Joao Aristeu ^[2] ; Chaboli Alevi, Kaio Cesar ^[2]

Affiliation

^[1] Univ Estadual Paulista, Lab Biol Celular, Inst Biociencias Letras & Ciencias Exatas, Dept Biol, IBILCE UNESP, Sao Jose Do Rio Preto - Brazil

^[2] Univ Estadual Paulista, Lab Parasitol, Dept Ciencias Biol, FCFAR UNESP, Fac Ciencias Farmaceut, Araraquara - Brazil

^[3] Univ Fed Piaui UFPO, Ctr Ciencias Saude, Dept Parasitol & Microbiol, Teresina - Brazil

^[4] Inst Oswaldo Cruz, Lab Biodivers Entomol, Rio De Janeiro - Brazil

• Document type: Journal article
• Source: American Journal of Tropical Medicine and Hygiene; v. 100, n. 4, p. 907-910, 2019.
• Web of Science Citations: 0

Abstract

Triatoma sordida is among the main Brazilian species considered as Chagas disease vectors. The genetic studies are directed mainly to phylogenetic questions because this species possibly have suffered cryptic speciation. Furthermore, there are few studies that analyzed the structure and genetic variability of specimens from Brazil and that showed low genetic diversity and strong genetic structuring of the population samples. Therefore, because of great epidemiological importance of T. sordida and mainly the restriction of genetic characterization of this vector only for populations of Minas Gerais state, this article performs a genetic analysis of the T. sordida from seven different Brazilian states (representing different biomes), by means of cytogenetic markers. All analyzed specimens presents the same cytogenetic characteristics: early meiotic prophase with several heterochromatic bodies dispersed in the nucleus (CGrich), being one of them formed by the associated sex chromosomes surrounded by some autosomal heterochromatic regions, meiotic metaphase with most autosomal pairs exhibiting a C-heterochromatic block in one chromosomal end (CG-rich), Y sex chromosome fully heterochromatin (AT-rich), and X chromosome may present a small C-block (CG-rich). These results are important because the chromosomal markers enable to confirm and expand the low genetic diversity for all Brazilian states occupied by T. sordida, suggesting that all Brazilian populations were originated from a small ancestral population and possibly dispersed to other biomes by founder effect. In addition, we suggest that T. sordida from Brazil are not suffering cryptic speciation and we confirm the classification of all Brazilian examples as T. sordida sensu stricto. (AU)

• FAPESP's process: 17/05015-7 - Study of hybridization in Triatoma sordida (Stal, 1859) (Hemiptera, Reduviidae): evolutionary dynamics, reproductive viability, morpho-molecular characterization and its taxonomic implications
• Grantee: Kaio Cesar Chaboli Alevi
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 18/12039-2 - Triatoma sordida (Stål, 1859) (Hemiptera, Triatominae): a Chagas Disease vector or a complex of vectors?
• Grantee: Isadora de Freitas Bittinelli
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 14/07706-9 - Chromosome analysis of different lineages of Triatoma sordida of Brazil, with emphasis on the phenomenon of cryptic speciation
• Grantee: Yago Visinho dos Reis
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 13/19764-0 - Cytotaxonomy and chromosomal evolution in Triatominae subfamily
• Grantee: Kaio Cesar Chaboli Alevi
• Support Opportunities: Scholarships in Brazil - Doctorate