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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Gold nanorods and poly(amido amine) dendrimer thin film for biosensing

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Author(s):
Ribovski, Lais [1] ; dos Santos, Fabricio A. [1] ; Zucolotto, Valtencir [1] ; Janegitz, Bruno C. [2]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Phys Inst Sao Carlos, Nanomed & Nanotoxicol Grp, CP 369, BR-13560970 Sao Carlos, SP - Brazil
[2] Univ Fed Sao Carlos, Dept Nat Sci Math & Educ, BR-13600970 Araras, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Journal of Solid State Electrochemistry; v. 23, n. 5, p. 1581-1591, MAY 2019.
Web of Science Citations: 0
Abstract

The use of gold nanomaterials in electrochemical biosensing has been proven to be effective either by modifying the electrodes' surface or by labeling molecules. The combination of dendrimers with gold nanomaterials is a worthwhile alternative to create a suitable environment to immobilize enzymes. In this paper, we report the development of a thin film composed of gold nanorods (AuNRs) and poly(amido amine) (PAMAM) dendrimer generation 4, which was applied for biosensing. The film was prepared by drop-casting the dispersion onto a screen-printed carbon electrode (SPCE), and tyrosinase (Tyr) enzyme was further immobilized onto the modified electrode. The direct electron transfer (DET) between the enzyme and electrode surface was verified through cyclic voltammetry (CV), yielding an apparent heterogeneous electron transfer rate constant of 0.045s(-1). Analytical curves were obtained by chronoamperometry for catechol (CAT) and dopamine (DA) with linear ranges from 2.8 to 30.3molL(-1) and 27.8 to 448.7molL(-1), respectively, and detection limits of 1.0molL(-1) for CAT and 10.0molL(-1) for DA. The improved electrochemical properties of AuNRs-PAMAM-modified SPCE combined with the effective enzyme immobilization led to a promising electrochemical device to detect phenolic compounds. (AU)

FAPESP's process: 15/19099-2 - Development of electrochemical sensors and biosensors for different analytical purposes
Grantee:Bruno Campos Janegitz
Support Opportunities: Regular Research Grants