Evaluation of Tagetes patula (Asteraceae) as an ecological alternative in the search for natural control of the cattle tick Rhipicephalus (Boophilus) microplus (Acari: Ixodidae)

Author(s)

Sanches Politi, Flavio Augusto ^[1] ; Fantatto, Rafaela Regina ^[2] ; da Silva, Alexander Alves ^[1] ; Moro, Isabela Jacob ^[2] ; Sampieri, Bruno Rodrigues ^[3] ; Camargo-Mathias, Maria Izabel ^[4] ; Figueiredo, Amanda ^[5] ; de Souza Chagas, Ana Carolina ^[5] ; Furlan, Maysa ^[1]

Affiliation

^[1] Univ Estadual Paulista Julio de Mesquita Filho UN, Inst Quim Araraquara, Araraquara, SP - Brazil

^[2] Univ Estadual Paulista Julio de Mesquita Filho UN, Fac Ciencias Farmaceut Araraquara, Araraquara, SP - Brazil

^[3] Univ Estadual Campinas, UNICAMP, Museu Zool, Campinas, SP - Brazil

^[4] Univ Estadual Paulista Julio de Mesquita Filho UN, Inst Biociencias Rio Claro, Rio Claro, SP - Brazil

^[5] Embrapa Pecuaria Sudeste, Empresa Brasileira Pesquisa Agr, Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: Experimental and Applied Acarology; v. 77, n. 4, p. 601-618, APR 2019.
• Web of Science Citations: 0

Abstract

The cattle tick Rhipicephalus microplus is a great sanitary problem and causes huge losses to livestock, being a vector of important diseases. The aim of this work was to verify the action of plant-derived material obtained from Tagetes patula on the life cycle of R. microplus, as well as to analyze the action of these extracts on ovary cells of engorged females. In the Adult Immersion Test, the crude ethanolic extract (Tp(EtOH)) showed an efficacy of 99.2% (LC50=18.60mgmL(-1)). From the repellency test, it was found that both the Tp(EtOH) and essential oil were 100% efficient on the larval stage. In addition, in the Larval Package Test, a mortality of 98.37% was obtained with Tp(EtOH) (LC50=3.798mgmL(-1)). From the microscopic analysis of ovary cells, morphological changes in the chorion and cytoplasm of oocytes were observed, with vacuolization around the germinal vesicle and disorganization of the pedicular cells, suggesting an interference in the normal embryogenic formation of the larvae. These results demonstrate that T. patula extracts interfere at all stages of development of the ixodid, from eggs to adults forms, and have a pronounced repellent effect. In addition, the results of the cytotoxicity assays performed on keratinocytes, as well as previous information on oral and dermal acute toxicity (LD50>4000mgkg(-1)), attest that T. patula can be a safe alternative for ectoparasitic control, representing an alternative for development of a formulation used to help control populations of ticks in the field. (AU)

• FAPESP's process: 13/07600-3 - CIBFar - Center for Innovation in Biodiversity and Drug Discovery
• Grantee: Glaucius Oliva
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 13/03493-8 - Potential of crude extracts, essential oil and pure compounds obtained from Tagetes patula L. (Asteraceae) as botanical biopesticide against Rhipicephalus (Boophilus) microplus, Haemonchus contortus and Cimex lectularis
• Grantee: Flávio Augusto Sanches Politi
• Support Opportunities: Scholarships in Brazil - Post-Doctoral