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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Human pegivirus-1 pegivirus-1 (HPgV-1, GBV-C) RNA prevalence and genotype diversity among volunteer blood donors from an intra-hospital hemotherapy service in Southern Brazil

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Author(s):
Slavov, Svetoslav Nanev [1, 2] ; Silveira, Roberta Maraninchi [1] ; Rodrigues, Evandra Strazza [1] ; Diefenbach, Cristiane Fracao [3] ; Zimmermann, Ana Maria [4] ; Covas, Dimas Tadeu [1] ; Kashima, Simone [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Blood Ctr Ribeirao Preto, BR-14051140 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Internal Med, BR-14051140 Ribeirao Preto, SP - Brazil
[3] Univ Fed Santa Maria, Univ Hosp Santa Maria, Hematol & Oncol Serv, BR-97105340 Santa Maria, RS - Brazil
[4] Hosp Dr Astrogildo de Azevedo, Hemotherapy Serv, BR-97015372 Santa Maria, RS - Brazil
Total Affiliations: 4
Document type: Journal article
Source: TRANSFUSION AND APHERESIS SCIENCE; v. 58, n. 2, p. 174-178, APR 2019.
Web of Science Citations: 2
Abstract

Objective: Human pegivirus (HPgV-1, GBV-C) is classified within the Pegivirus genus of the Flaviviriade family. The natural history of HPgV-1 infection is still unclear, however, the main route of viral transmission seems to be the parenteral one. The detection of HPgV-1 viremia in blood donors without parenteral exposure demonstrates that other routes of HPgV-1 transmission might also exist. The objective of the present study was to evaluate the prevalence of HPgV-1 RNA and circulating genotypes among blood donors from a intra-hospital Hemotherapy Service localized in the Santa Maria city, central part of the Rio Grande do Sul State in the extreme South of Brazil. Methods: Blood samples were obtained from 373 volunteer blood donors and tested for the presence of HPgV-1 RNA. All positive for RNA samples were submitted to sequencing and phylogenetic analysis. Results: The prevalence of the HPgV-1 RNA was 5.9% (22/373). The performed phylogenetic analysis demonstrated a predominant detection of genotype 2 with its both subgenotype forms (95.5% of all isolates i.e 54.5% belonging to subgenotype 2 A and 40.9% belonging to subgenotype 2B). Only one sequence was classified as genotype 3 (1/22, 4.5%). Conclusions: Our study demonstrates the circulation pattern and genotypes of HPgV-1 among volunteer blood donors of South Brazil, and adds to the global knowledge of the natural history and possible transmission routes of this viral agent with putative impact on the area of hemotherapy. (AU)

FAPESP's process: 14/50947-7 - INCT 2014: in Stem Cell and Cell Therapy
Grantee:Dimas Tadeu Covas
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 17/23205-8 - Evaluation of the impact of emerging and reemerging viruses in the field of hemotherapy and stem cell transplantation by multiple-research approach
Grantee:Svetoslav Nanev Slavov
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 09/16623-1 - Molecular pathogenesis and clinical consequences of the infections caused by the Human Parvovirus B19 and Human Cytomegalovirus among patients with hemaglobinopathies and haemophilia
Grantee:Svetoslav Nanev Slavov
Support Opportunities: Scholarships in Brazil - Post-Doctoral