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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Losartan and isoproterenol promote alterations in the local renin-angiotensin system of rat salivary glands

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Cano, Isadora Prado [1] ; Dionisio, Thiago Jose [2] ; Cestari, Tania Mary [2] ; Calvo, Adriana Maria [2] ; Colombini-Ishikiriama, Bella Luna [2] ; Cardoso Faria, Flavio Augusto [3, 4] ; Siqueira, Walter Luiz [3, 4] ; Santos, Carlos Ferreira [2]
Total Authors: 8
[1] Univ Sao Paulo, Bauru Sch Dent, Dept Prosthodont & Periodontol, Bauru, SP - Brazil
[2] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Bauru, SP - Brazil
[3] Univ Western Ontario, Schulich Sch Med & Dent, Dept Biochem, London, ON - Canada
[4] Univ Western Ontario, Schulich Sch Med & Dent, Sch Dent, London, ON - Canada
Total Affiliations: 4
Document type: Journal article
Source: PLoS One; v. 14, n. 5 MAY 22 2019.
Web of Science Citations: 0

Renin-angiotensin system (RAS) systemically or locally collaborates with tissue homeostasis, growth and development, which has been extensively studied for its pharmacological implications. This study was primarily aimed at finding and characterizing local RAS in rat parotid, sublingual and submandibular glands. It was also hypothesized that vasoactive drugs could affect the expression of RAS targets, as well as saliva flow and its composition. Therefore, another objective of this study was to compare the effects of losartan (angiotensin II receptor blocker) and isoproterenol (beta-adrenergic receptor agonist). Forty-one Wistar rats were divided into three groups and administered a daily intraperitoneal dose of saline, losartan or isoproterenol solutions for one week. The following RAS targets were studied using qPCR: renin (REN), angiotensinogen (AGT), angiotensin converting enzyme (ACE), ACE-2, elastase-2 (ELA-2), AT1-a and MAS receptors, using RPL-13 as a reference gene. Morphology of glands was analyzed by immunohistochemistry using REN, ACE, ACE-2, AT1, AT2 and MAS antibodies. The volume and total protein content of saliva were measured. Our results revealed that ACE, ACE-2, AT1-a, AT2 and MAS receptors were expressed in all salivary gland samples, but REN and ELA-2 were absent. Losartan decreased mRNA expression of RAS targets in parotid (MAS) and submandibular glands (ACE and both AT receptors), without affecting morphological alterations, and significantly decreased saliva and total protein secretions. Isoproterenol treatment affected gene expression profiles in parotid (ACE, ACE-2, AT1-a, MAS, AGT), and submandibular (ACE, AT2, AGT) glands, thus promoting acinar hypertrophy in serous acini, without significant changes in salivary flow or total protein content. These drugs affected mainly acini, followed by duct systems and myoepithelial cells, whereas blood vessels were not affected. In conclusion, there is a local RAS in major rat salivary glands and losartan, an angiotensin II receptor blocker, affected not only the RAS-target gene expression but also decreased salivary flow and total protein content. (AU)

FAPESP's process: 14/12975-9 - The local renin-angiotensin system in rat salivary glands: pharmacological alterations
Grantee:Isadora Prado Cano
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 14/22379-4 - The local renin-angiotensin system in rat salivary glands: pharmacological alterations
Grantee:Isadora Prado Cano
Support type: Scholarships abroad - Research Internship - Scientific Initiation
FAPESP's process: 17/17747-2 - Qualitative and quantitative analysis of gingival sulcus cytokines in teeth chosen for metal ceramic crowns during different steps of clinical procedures, in patients taking Angiotensin II AT1a receptor antagonists
Grantee:Isadora Prado Cano
Support type: Scholarships in Brazil - Master
FAPESP's process: 15/03965-2 - Role of the renin-angiotensin system in different oral inflammatory models: an experimental interdisciplinary and clinical approach
Grantee:Carlos Ferreira dos Santos
Support type: Research Projects - Thematic Grants