Analysis of copper response in Acinetobacter sp. by comparative proteomics

Author(s)

Gracioso, Louise Hase ^{[1, 2]} ; Galluzzi Baltazar, Marcela Passos ^{[1, 3]} ; Avanzi, Ingrid Regina ^[1] ; Karolski, Bruno ^{[1, 3]} ; Oller Nascimento, Claudio Augusto ^[3] ; Perpetuo, Elen Aquino ^{[1, 4]}

Affiliation

^[1] Univ Sao Paulo, Environm Res & Educ Ctr, CEPEMA, POLI, Conego Domen Rangoni Rd, 270 Km, Cubatao, SP - Brazil

^[2] Univ Sao Paulo, Interunits Grad Program Biotechnol, Lineu Prestes Ave 2415, Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Dept Chem Engn, POLI, Lineu Prestes Ave 580, Sao Paulo, SP - Brazil

^[4] Univ Fed Sao Paulo, Dept Marine Sci, Imar, Unifesp, Carvalho Mendonca Ave 144, Sao Paulo - Brazil

• Document type: Journal article
• Source: METALLOMICS; v. 11, n. 5, p. 949-958, MAY 1 2019.
• Web of Science Citations: 0

Abstract

Metal contamination exerts environmental pressure on several lifeforms. Since metals are non-biodegradable and recalcitrant, they accumulate in living beings and spread through the food chain. Thus, many life forms are affected by environmental metal contamination, such as plants and microorganisms. In the case of microorganisms, scarce information is available on how metals affect them. As a highly resistant form of life, microorganisms can adapt to several environmental pressures through genetic modifications, changing their metabolism to overcome new conditions, and continuing to thrive in the same place. In this study, an Acinetobacter sp. strain was isolated from a copper mine, which presented very high resistance to copper, growing in copper concentrations of up to 7 mM. As a result of its metabolic response in the presence of 3 mM of copper, the expression of 35 proteins in total was altered. The proteins were identified to be associated with the glycolytic pathway, membrane transport, biosynthesis and two proteins directly involved in copper homeostasis (CopA and CopB). (AU)

• FAPESP's process: 12/06600-7 - Study on microbial activity in bioremediation of heavy metals in Amazon area
• Grantee: Ingrid Regina Avanzi
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 13/50218-2 - CEPEMA - Cooperative Center of Environmental Engineering
• Grantee: Claudio Augusto Oller do Nascimento
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 13/11020-2 - Characterization of the bacterial community of a copper processing and identification of heavy metals resistance mechanism
• Grantee: Louise Hase Gracioso
• Support Opportunities: Scholarships in Brazil - Doctorate