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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Fibroblast growth factor 2 regulates cumulus differentiation under the control of the oocyte

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Author(s):
Barros, Rodrigo G. [1] ; Lima, Paula F. [1] ; Soares, Ana Caroline S. [1] ; Sanches, Lorena [1] ; Price, Christopher A. [2] ; Buratini, Jose [1]
Total Authors: 6
Affiliation:
[1] Univ Estadual Paulista, Dept Fisiol, Inst Biociencias, BR-18618970 Sao Paulo - Brazil
[2] Univ Montreal, Fac Med Vet, Ctr Rech Reprod & Fertilite, St Hyacinthe, PQ J2S 7C6 - Canada
Total Affiliations: 2
Document type: Journal article
Source: JOURNAL OF ASSISTED REPRODUCTION AND GENETICS; v. 36, n. 5, p. 905-913, MAY 2019.
Web of Science Citations: 0
Abstract

PurposeWe first assessed regulation of FGF2 expression in cumulus cells by FSH and oocyte-secreted factors during in vitro maturation (IVM). Then, we tested the hypothesis that FGF2 regulates meiotic progression, cumulus expansion, and apoptosis in cumulus-oocyte complexes (COC) undergoing IVM.MethodsIn vitro maturation of bovine COC was utilized as a model to assess regulation of FGF2 expression by FSH and oocyte-secreted factors (via microsurgical removal of the oocyte), as well as effects of graded doses of FGF2 on meiotic progression, degree of cumulus expansion, dissociation of cumulus cells, and cumulus cells apoptosis. Expression of genes regulating functional endpoints altered by FGF2 treatment was assessed in cumulus cells by real-time PCR. Cultures were replicated 4-5 times and effects of treatments were tested by ANOVA.ResultsFGF2 mRNA expression was increased by FSH and oocyte-secreted factors during IVM. Addition of FGF2 to the IVM medium advanced meiosis resumption, decreased the ease with which cumulus cells were dissociated, and inhibited cumulus cells apoptosis. Decreased cumulus dissociation was accompanied by decreased expression of TNFAIP6.ConclusionsThis is the first study showing that FGF2 expression is regulated by the oocyte in cumulus cells. Moreover, we report novel effects of FGF2 on cumulus cell survival and extracellular matrix (ECM) quality during IVM that may favor acquisition of developmental competence and suggest physiological roles during the final steps of COC differentiation. (AU)

FAPESP's process: 12/06417-8 - Fibroblast growth factors (FGFs) in the differentiation of the bovine cumulus-oocyte complex
Grantee:José Buratini Junior
Support Opportunities: Regular Research Grants