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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Evaluation of antileishmanial drugs activities in an ex vivo model of leishmaniasis

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Author(s):
Salazar Terreros, Myriam Janeth [1] ; Visani de Luna, Luis Augusto [1, 2] ; Giorgio, Selma [1]
Total Authors: 3
Affiliation:
[1] Univ Estadual Campinas, Inst Biol, Dept Anim Biol, Campinas, SP - Brazil
[2] Univ Estadual Campinas, Inst Chem, Lab Solid State Chem, Campinas, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Parasitology International; v. 71, p. 163-166, AUG 2019.
Web of Science Citations: 0
Abstract

Leishmaniasis is a poverty-related disease, the chemotherapy of which is based on few drugs. The in vitro macrophage-amastigote model using mouse peritoneal cells, human-monocyte transformed macrophages and immortalized cell lines have been used to test new and safe antileishmanial drugs. Considering the differences for drug sensitivities between these Leishmania infected cells, the efficacy of amphotericin B, pentavalent antimonial, miltefosine and resveratrol was evaluated in a recently developed ex vivo culture of macrophages isolated from mouse lesion induced by L. amazonensis (CD11b(+)F4/80(+)CD68(+)CD14(+)) compared with infected peritoneal macrophages (CD11b(+)F4/80(+)CD68(+)CD14(+)). The results show that IC50 values of amphotericin B, miltefosine and pentavalent antimonial for parasites in lesional and peritoneal macrophages were similar, although high doses of these compounds did not result in total clearance of parasites in lesional cells (amphotericin B), peritoneal cells (miltefosine) and both cell cultures (pentavalent antimonial). Amastigotes infecting lesional macrophages were more resistant to resveratrol as compared to parasites in peritoneal macrophages. The cytoxicity of miltefosine and resveratrol was higher in infected peritoneal macrophages than in lesional cells. These data suggest that the antileishmanial effect and citotoxicity of some anti leishmanial compounds are dependent of macrophage source and mouse peritoneal macrophages loaded with amastigotes do not represent the lesion cell. (AU)

FAPESP's process: 18/23302-6 - Leishmaniasis of medical importance in Brazil: study of cellular systems and clinical isolates of Leishmania infantum and L. braziliensis and evaluation of vaccine candidates against L. infantum
Grantee:Selma Giorgio
Support Opportunities: Regular Research Grants