| Full text | |
| Author(s): Show less - |
Toledo, Beatriz
[1]
;
Corral, Marcelo A.
[1]
;
Meisel, Dirce Mary C. L.
[1]
;
Gottardi, Maiara
[1]
;
Abdala, Edson
[2]
;
Costa, Silvia F.
[2]
;
Pierrotti, Ligia Camera
[2]
;
Lescano, Susana A. Z.
[3, 1]
;
Goncalves, Elenice M. N.
[4]
;
Castilho, Vera L. P.
[4]
;
Chieffi, Pedro P.
[5]
;
Gryschek, Ronaldo C. B.
[3, 1]
;
Paula, Fabiana M.
[3, 1]
Total Authors: 13
|
| Affiliation: | [1] Univ Sao Paulo, Fac Med, Hosp Clin HCFMUSP, Lab Invest Med LIM 06, Lab Imunopatol Esquistossom, Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Fac Med, Hosp Clin HCFMUSP, Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Inst Med Trop Sao Paulo, Sao Paulo, SP - Brazil
[4] Univ Sao Paulo, Fac Med, Hosp Clin HCFMUSP, Secao Parasitol, Div Lab Cent, Sao Paulo, SP - Brazil
[5] Fac Ciencias Med, Sao Paulo, SP - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | Clinics; v. 74, 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
OBJECTIVES: Hyperinfection or disseminated strongyloidiasis has been frequently reported after transplants and is related to high mortality. This study aimed to screen for strongyloidiasis using serological diagnoses in transplant candidates. METHODS: An ELISA test was performed with filariform larvae of Strongyloides venezuelensis as a source of antigen. RESULTS: In the serum from transplant candidates, anti-Strongyloides IgG antibodies were detected in 35/150 (23.3%) samples by soluble fractions in phosphate buffered saline (PBS), 31/150 (20.7%) samples by soluble fractions in Tris-HCl, 27/150 (18.0%) samples by membrane fractions in PBS and 22/150 (14.7%) samples by membrane fractions in Tris-HCl. CONCLUSIONS: The present results suggest the ELISA test, ideally using soluble fractions of filariform larvae S. venezuelensis in PBS, as an additional strategy for the diagnosis of strongyloidiasis in transplant candidates. (AU) | |
| FAPESP's process: | 13/04236-9 - Immunoproteomics applied to the diagnosis human strongyloidiasis used antigen Strongyloides venezuelensis |
| Grantee: | Ronaldo Cesar Borges Cryschek |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 10/51110-2 - Investigation of diagnostic techniques for identification of infection S. stercoratis in patients with different forms of immunosuppression |
| Grantee: | Pedro Paulo Chieffi |
| Support Opportunities: | Regular Research Grants |