Williamsia aurantiacus sp. nov. a novel actinobacterium producer of antimicrobial compounds isolated from the marine sponge

Author(s)

Afonso de Menezes, Claudia Beatriz ^{[1, 2]} ; Afonso, Rafael Sanches ^[1] ; de Souza, Wallace Rafael ^[3] ; Parma, Marcia Maria ^[3] ; de Melo, Itamar Soares ^[3] ; Satoru Fugita, Fernando Lucas ^[4] ; Beraldo Moraes, Luiz Alberto ^[4] ; Zucchi, Tiago Domingues ^[5] ; Fantinatti-Garboggini, Fabiana ^{[1, 2]}

Affiliation

^[1] Univ Estadual Campinas, Ctr Pluridisciplinar Pesquisas Quim Biol & Agr, Av Alexandre Cazelatto 999, BR-13148218 Paulinia, SP - Brazil

^[2] Univ Estadual Campinas, Programa Posgrad Genet & Biol Mol, CP 6109, BR-13083970 Campinas, SP - Brazil

^[3] Embrapa Meio Ambiente, Rodovia SP 340 Km 127-5, CP 69, BR-13820000 Jaguariuna, SP - Brazil

^[4] Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, Av Bandeirantes 3900, BR-12040901 Ribeirao Preto, SP - Brazil

^[5] Agrivalle, Av Tranquillo Giannini, BR-13329600 Salto, SP - Brazil

• Document type: Journal article
• Source: Archives of Microbiology; v. 201, n. 5, p. 691-698, JUL 2019.
• Web of Science Citations: 0

Abstract

An antibiotic-producing actinobacterium, designated isolate B375(T), was isolated from marine sponge Glodia corticostylifera collected from Praia Guaeca, SAo Paulo, Brazil (23 degrees 49S; 45 degrees 25W), and its taxonomic position established using data from a polyphasic study. The organism showed a combination of morphological, physiological, biochemical and chemotaxonomic characteristics consistent with its classification in the genus Williamsia. Comparative 16S rRNA gene sequence analysis indicated that the strain B375(T) was most closely related to Williamsia serinedens DSM 45037(T) and Williamsia spongiae DSM 46676(T) and having 99.43% and 98.65% similarities, respectively, but was distinguished from these strains by a low level of DNA-DNA relatedness (53.2-63.2%) and discriminatory phenotypic properties. Chemotaxonomic investigations revealed the presence of cell-wall chemotype IV and N-glycolated muramic acid residues present in the wall cells. The cells contained C-16:0 (23.3%), C-18:0 10-methyl (23.2%) and C-18:1 9c (21.6%) as the major cellular fatty acids. The strain B375(T) inhibited growing of Staphylococcus aureus and Colletotrichum gloeosporioides strains and was considered a producer of antimicrobial compounds. Based on the data obtained, the isolate B375(T) (=CBMAI 1090(T)=DSM 46677(T)) should, therefore, be classified as the type strain of a novel species of the genus Williamsia, for which the name Williamsia aurantiacus sp. nov. is proposed. (AU)

• FAPESP's process: 16/22023-0 - Activation of the Cryptic Secondary Metabolite Biosynthetic Gene Clusters in Actinomycetes by Antibiotics Resistance
• Grantee: Luiz Alberto Beraldo de Moraes
• Support Opportunities: Regular Research Grants