In vitro methods to determine the antioxidant activity of caffeic acid

Author(s)

Spagnol, Caroline Magnani ^[1] ; Assis, Renata Pires ^[2] ; Brunetti, Iguatemy Lourenco ^[2] ; Borges Isaac, Vera Lucia ^[1] ; Nunes Salgado, Herida Regina ^[1] ; Correa, Marcos Antonio ^[1]

Affiliation

^[1] Sao Paulo State Univ UNESP, Dept Drugs & Med, Sch Pharmaceut Sci, Rod Araraquara Jau Km 1, Campus Ville, BR-14800903 Araraquara, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Dept Clin Anal, Sch Pharmaceut Sci, BR-14800903 Araraquara, SP - Brazil

• Document type: Journal article
• Source: SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY; v. 219, p. 358-366, AUG 5 2019.
• Web of Science Citations: 1

Abstract

Caffeic acid (CA) is a plant metabolite acting as a carcinogenic inhibitor, and exhibits a high antioxidant effect and some antimicrobial activity. Besides, this compound can be useful in the prevention of heart diseases and atherosclerosis, among others. The present study aims to determine the in vitro antioxidant activity of CA in order to increase the frequency of its use and reliability in the prevention of damage caused by free radicals and other reactive species. The tests performed were as follows: Radical anion superoxide capture; crocin bleaching assay: capturing ability of hypochlorous add; H2O2 capture; capturing capacity of the ABTS(center dot+)/DPPH center dot; and SOD-like activity. The values of the CA antioxidant activity were very close to the values of standards in all tests. Besides, CA presented an antioxidant activity greater than that of ascorbic acid and trolox, and its advantages include higher stability than ascorbic add and extraction from natural sources, as opposed to trolox. (C) 2019 Published by Elsevier B.V. (AU)

• FAPESP's process: 15/02619-3 - Biodegradable polymeric microparticles containing caffeic acid for dermal application
• Grantee: Caroline Magnani Spagnol
• Support Opportunities: Scholarships in Brazil - Doctorate