| Full text | |
| Author(s): Show less - |
Tessarin, Gestter W. L.
[1, 2]
;
Michalec, Ola M.
[3]
;
Torres-da-Silva, Kelly R.
[1, 2]
;
Da Silva, V, Andre
;
Cruz-Rizzolo, Roelf J.
[1]
;
Goncalves, Alaide
[1]
;
Gasparini, Daniele C.
[1]
;
Horta-Junior, Jose A. C.
[4]
;
Ervolino, Edilson
[1]
;
Bittencourt, Jackson C.
[5]
;
Lovejoy, David A.
[3]
;
Casatti, Claudio A.
[1, 4]
Total Authors: 12
|
| Affiliation: | [1] Sao Paulo State Univ UNESP, Dept Basic Sci, Sch Dent Aracatuba, Aracatuba - Brazil
[2] Sao Paulo State Univ UNESP, Dept Anat, Inst Biosci Botucatu, Botucatu, SP - Brazil
[3] Univ Toronto, Dept Cell & Syst Biol, Toronto, ON - Canada
[4] Da Silva, Andre, V, Sao Paulo State Univ UNESP, Dept Anat, Inst Biosci Botucatu, Botucatu, SP - Brazil
[5] Sao Paulo Univ USP, Dept Anat, Inst Biomed Sci, Sao Paulo - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | FRONTIERS IN NEUROSCIENCE; v. 13, JUN 27 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
Teneurins are type II transmembrane proteins comprised of four phylogenetically conserved homologs (Ten-1-4) that are highly expressed during neurogenesis. An additional bioactive peptide named teneurin C-terminal-associated peptide (TCAP-1-4) is present at the carboxyl terminal of teneurins. The possible correlation between the Ten/TCAP system and brain injuries has not been explored yet. Thus, this study examined the expression of these proteins in the cerebral cortex after mechanical brain injury. Adult rats were subjected to cerebral cortex injury by needle-insertion lesion and sacrificed at various time points. This was followed by analysis of the lesion area by immunohistochemistry and conventional RT-PCR techniques. Control animals (no brain injury) showed only discrete Ten-2-like immunoreactive pyramidal neurons in the cerebral cortex. In contrast, Ten-2 immunoreactivity was significantly up-regulated in the reactive astrocytes in all brain-injured groups (p < 0.0001) when compared to the control group. Interestingly, reactive astrocytes also showed intense immunoreactivity to LPHN-1, an endogenous receptor for the Ten-2 splice variant named Lasso. Semi-quantitative analysis of Ten-2 and TCAP-2 expression revealed significant increases of both at 48 h, 3 days and 5 days (p < 0.0001) after brain injury compared to the remaining groups. Immortalized cerebellar astrocytes were also evaluated for Ten/TCAP expression and intracellular calcium signaling by fluorescence microscopy after TCAP-1 treatment. Immortalized astrocytes expressed additional Ten/TCAP homologs and exhibited significant increases in intracellular calcium concentrations after TCAP-1 treatment. This study is the first to demonstrate that Ten-2/TCAP-2 and LPHN-1 are upregulated in reactive astrocytes after a mechanical brain injury. Immortalized cerebellar astrocytes expressed Ten/TCAP homologs and TCAP-1 treatment stimulated intracellular calcium signaling. These findings disclose a new functional role of the Ten/TCAP system in astrocytes during tissue repair of the CNS. (AU) | |
| FAPESP's process: | 09/54141-9 - Acquisition of a confocal laser scanning microscopy system and a transmission electron microscope for the auxiliary Electron Microscopy Center |
| Grantee: | Silvia Rodrigues Machado |
| Support Opportunities: | Multi-user Equipment Program |
| FAPESP's process: | 13/26779-4 - Effect of zoledronate during the alveolar healing process in rats: study involving the major risk factors for developing osteonecrosis of the jaws and assessment of antimicrobial photodynamic therapy (aPDT) as preventive therapy |
| Grantee: | Edilson Ervolino |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 10/52068-0 - The putative envolvement of melanin-concentrating hormone in the control of lactation |
| Grantee: | Jackson Cioni Bittencourt |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 12/08833-9 - Expression of teneurins and teneurins C-terminal associated peptides (TCAPs) in the central nervous system of non-human primates (Cebus apella) |
| Grantee: | Kelly Regina Torres da Silva |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 08/02771-6 - Connectivity, neurochemistry and behavioural evaluation of afferents to the first nucleus of the acoustic startle reflex neural circuit: the cochlear root nucleus |
| Grantee: | José de Anchieta de Castro e Horta Júnior |
| Support Opportunities: | Research Grants - Young Investigators Grants |