Obesity as an Inflammatory Agent Can Cause Cellular Changes in Human Milk due to the Actions of the Adipokines Leptin and Adiponectin

Author(s)

Morais, Tassiane C. ^{[1, 2]} ; de Abreu, Luiz C. ^{[1, 2, 3]} ; de Quental, Ocilma B. ^[2] ; Pessoa, Rafael S. ^{[2, 4]} ; Fujimori, Mahmi ^{[2, 4]} ; Daboin, Blanca E. G. ^[2] ; Franca, Eduardo L. ^{[2, 4]} ; Honorio-Franca, Adenilda C. ^{[2, 4]}

Affiliation

^[1] Univ Sao Paulo, Sch Publ Hlth, Postgrad Program Publ Hlth, BR-01246904 Sao Paulo, SP - Brazil

^[2] Ctr Univ SaUde ABC FMABC, Dept Morphol & Physiol, Lab Sci Writing, BR-09060870 Santo Andre, SP - Brazil

^[3] Sch Sci Santa Casa Misericordia Vitoria EMESCAM, Postgrad Program Publ Policies & Local Dev, BR-29045402 Vitoria, ES - Brazil

^[4] Fed Univ Mato Grosso UFMT, Inst Biol & Hlth Sci, BR-78600000 Mato Grosso, MT - Brazil

• Document type: Journal article
• Source: CELLS; v. 8, n. 6 JUN 2019.
• Web of Science Citations: 2

Abstract

Adiponectin and leptin play roles in the hunger response, and they can induce the inflammatory process as the initial mechanism of the innate immune response. It is possible for alterations in the levels of these adipokines to compromise the functional activity of human colostrum phagocytes. Therefore, the objective of this study is to analyze the effects of adiponectin and leptin on colostrum mononuclear (MN) cells. Colostrum was collected from 80 healthy donors, who were divided into two groups: the control group and the high body mass index (BMI) group. MN cells were used to analyze phagocytosis by flow cytometry, and reactive oxygen species (ROS), intracellular calcium, and apoptosis were assessed by fluorimetry using a microplate reader. Adipokines restored the levels of phagocytosis to the high BMI group (p < 0.05), with a mechanism that is action-dependent on the release of ROS and intracellular calcium. However, adiponectin and leptin simultaneously contributed to better microbicidal activity, thus reflecting an increase in the apoptosis level (p < 0.05) in the high BMI group. Probably, the maintenance of the balance between adiponectin and leptin levels enhances the protection and decreases the indices of neonatal infection in the breastfeeding infants of women with high BMI values. Therefore, policies that support pre-gestational weight control should be encouraged. (AU)

• FAPESP's process: 15/01051-3 - FUNCTIONAL ACTIVITY OF CELL MONONUCLEAR COLOSTRUM MOTHERS WITH OVERWEIGHT AND OBESITY AND THE ROLE OF AGENTS IMMUNOMODULATORS: ADIPONECTIN, LEPTIN AND MELATONIN
• Grantee: Tassiane Cristina Morais
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 15/19922-0 - Functional activity of mononuclear cells of colostrum of mothers with overweight and obesity and the role of immunomodulators agents: adiponectin, leptin and melatonin.
• Grantee: Luiz Carlos de Abreu
• Support Opportunities: Regular Research Grants