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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A fast and simple approach for the quantification of 40 illicit drugs, medicines, and pesticides in blood and urine samples by UHPLC-MS/MS

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Author(s):
Franco de Oliveira, Sarah C. W. S. E. [1, 2] ; Zucoloto, Alexandre D. [3, 2] ; de Oliveira, Carolina D. R. [3] ; Hernandez, Edna M. M. [3] ; Fruchtengarten, Ligia V. G. [3] ; de Oliveira, Tiago F. [1] ; Yonamine, Mauricio [2]
Total Authors: 7
Affiliation:
[1] Fed Univ Hlth Sci Porto Alegre, Pharmacosci Dept, Porto Alegre, RS - Brazil
[2] Univ Sao Paulo, Sch Pharmaceut Sci, Av Prof Lineu Prestes 580, 13B, BR-05508000 Sao Paulo, SP - Brazil
[3] Poison Control Ctr Sao Paulo, Sao Paulo, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Journal of Mass Spectrometry; v. 54, n. 7, p. 600-611, JUL 2019.
Web of Science Citations: 0
Abstract

A fast and simple approach to overcome challenges in emergency toxicological analysis, using ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) has been developed, for the detection of analytes in blood and urine samples from the following drug classes: analgesics, benzodiazepines, antidepressants, anticonvulsants, drugs of abuse, and pesticides. These substances are relevant in the context of emergency toxicology in Brazil. The sample preparation procedure was relatively easy and fast to perform. The method was fully validated giving limits of in the range of 0.5 and 20 ng mL(-1) for blood and urine samples. The intraday and interday precision and accuracy were considered adequate for all analytes once the relative standard deviation (RSD) (%) was lower than 20% for quality control (QC) low and lower than 15% for CQ medium and high. The developed method was successfully applied to 320 real samples collected at the Poison Control Center of Sao Paulo, and 89.1% have shown to be positive for some of the analytes. This confirms its applicability and importance to emergency toxicological analysis, and it could be very useful in both fields of clinical and forensic toxicology. (AU)

FAPESP's process: 14/05877-0 - Application of multivariate statistical techniques for the mapping of the cases of intoxication attended in poison control center in São Paulo City
Grantee:Sarah Carobini Werner de Souza Eller Franco de Oliveira
Support Opportunities: Scholarships in Brazil - Doctorate