Stability of medicines, drugs of abuse and pesticides in dried blood spots (DBS), and its application in forensic toxicology

• Grant number: 15/10650-8
• Support Opportunities: Regular Research Grants
• Duration: November 01, 2015 - January 31, 2018
• Field of knowledge: Health Sciences - Pharmacy - Toxicological Analysis
• Principal Investigator: José Luiz da Costa
• Grantee: José Luiz da Costa
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated researchers: Marina Franco Maggi Tavares

Abstract

In clinical and analytical toxicology, the availability of sophisticated analytical tools does not guarantee the quality of the result. The sample collection and processing represent a major challenge, since the errors that can occur during these steps possibly have great influence on the final analytical result. Dried blood spot (DBS) has enjoyed growing popularity as an alternative sampling procedure in the bioanalytical, clinical and pharmaceutical field, but few studies were dedicated to its use in forensic toxicology. DBS reproduce the composition of whole blood, but their sampling is much faster, less complex and less invasive. Furthermore, the DBS sample process does not require the use of anticoagulant or plasma separation and they can be stored for months at room temperature without any appreciable analyte loss. Lastly, the DBS process reduces the risk of infection by pathogens such as HIV or hepatitis, to a minimum. The aim of this project is to evaluate the stability of medicines, drug abuse and pesticides in DBS (prepared with post mortem blood), and compare the findings to the whole blood stability, stored at fridge. It will be studied 28 substances, most frequently identified at Forensic Toxicology Center (Legal Medical Institute of São Paulo), and also substances that, according to the literature, have low stability in blood and / or urine. (AU)

Scientific publications

(11)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA CUNHA, KELLY FRANCISCO; EBERLIN, MARCOS NOGUEIRA; COSTA, JOSE LUIZ. Development and validation of a sensitive LC-MS/MS method to analyze NBOMes in dried blood spots: evaluation of long-term stability. Forensic Toxicology, v. 36, n. 1, p. 113-121, JAN 2018. (15/10650-8)

DA CUNHA, KELLY FRANCISCO; EBERLIN, MARCOS NOGUEIRA; COSTA, JOSE LUIZ. Long-term stability of synthetic cathinones in dried blood spots and whole blood samples: a comparative study. Forensic Toxicology, v. 36, n. 2, p. 424-434, JUL 2018. (15/10650-8)

DE MORAIS, DAMILA RODRIGUES; BARBOSA, INGRID LOPES; CUNHA, KELLY FRANCISCO; TRIPODI, GUILHERME LUCAS; FIGUEIREDO ANGOLINI, CELIO FERNANDO; FRANCO, MARCOS FERNANDO; DE AQUINO, ELVIS MEDEIROS; EBERLIN, MARCOS NOGUEIRA; COSTA, JOSE LUIZ. EASI-IMS an expedite and secure technique to screen for 25I-NBOH in blotter papers. Journal of Mass Spectrometry, v. 52, n. 10, p. 701-706, OCT 2017. (16/23157-0, 15/10650-8)

CATA-PRETA, ELISANGELA G.; SERRA, YASMIM A.; MOREIRA-JUNIOR, ELISEU DA C.; REIS, HENRIQUE S.; KISAKI, NATALI D.; LIBARINO-SANTOS, MATHEUS; SILVA, RAIANY R. R.; BARROS-SANTOS, THAISA; SANTOS, LUCAS C.; BARBOSA, PAULO C. R.; et al. Ayahuasca and Its DMT- and beta-carbolines - Containing Ingredients Block the Expression of Ethanol-Induced Conditioned Place Preference in Mice: Role of the Treatment Environment. FRONTIERS IN PHARMACOLOGY, v. 9, MAY 29 2018. (15/10650-8)

RODRIGUES DE MORAIS, DAMILA; CAMPELO, JACQUELINE DE MORAIS; RAZZO, DANIEL; EBERLIN, MARCOS NOGUEIRA; COSTA, JOSE LUIZ; SANTOS, JANDYSON MACHADO. Forensic determination of crossing lines involving stamp and pen inks by mass spectrometry imaging. ANALYTICAL METHODS, v. 12, n. 7, p. 951-958, FEB 21 2020. (18/00432-1, 15/10650-8)

ANDRIGUETTI, NATALIA B.; HAHN, ROBERTA Z.; LIZOT, LILIAN F.; RAYMUNDO, SUZIANE; COSTA, JOSE L.; DA CUNHA, KELLY F.; VILELA, RAMON M. M.; KLUCK, HELENA M.; SCHWARTSMANN, GILBERTO; ANTUNES, MARINA V.; et al. Analytical and clinical validation of a dried blood spot assay for the determination of paclitaxel using high-performance liquid chromatography-tandem mass spectrometry. CLINICAL BIOCHEMISTRY, v. 54, p. 123-130, APR 2018. (15/10650-8)

DA CUNHA, KELLY FRANCISCO; EBERLIN, MARCOS NOGUEIRA; HUESTIS, MARILYN A.; COSTA, JOSE LUIZ. NBOMe instability in whole blood. Forensic Toxicology, v. 37, n. 1, p. 82-89, JAN 2019. (18/00432-1, 15/10650-8)

DE MORAIS, DAMILA RODRIGUES; LANARO, RAFAEL; BARBOSA, INGRID LOPES; SANTOS, JANDYSON MACHADO; CUNHA, KELLY FRANCISCO; HERNANDES, VINICIUS VERI; TESSARO, ELIAS PAULO; GOMES, CEZAR SILVINO; EBERLIN, MARCOS NOGUEIRA; COSTA, JOSE LUIZ. Ayahuasca and Kambo intoxication after alternative natural therapy for depression, confirmed by mass spectrometry. Forensic Toxicology, v. 36, n. 1, p. 212-221, JAN 2018. (15/10650-8, 16/23157-0)

GISELE A. B. CANUTO; JOSÉ LUIZ DA COSTA; PEDRO L. R. DA CRUZ; ANA ROSA L. DE SOUZA; ANDRÉA T. FACCIO; ALINE KLASSEN; KARINA T. RODRIGUES; MARINA F. M. TAVARES. METABOLÔMICA: DEFINIÇÕES, ESTADO-DA-ARTE E APLICAÇÕES REPRESENTATIVAS. Química Nova, v. 41, n. 1, p. 75-91, 2018-01-00. (12/09822-0, 15/10650-8, 12/51692-7, 12/07361-6)

COSTA, JOSE LUIZ; CUNHA, KELLY FRANCISCO; LANARO, RAFAEL; CUNHA, RICARDO LEAL; WALTHER, DONNA; BAUMANN, MICHAEL H.. Analytical quantification, intoxication case series, and pharmacological mechanism of action for N-ethylnorpentylone (N-ethylpentylone or ephylone). DRUG TESTING AND ANALYSIS, v. 11, n. 3, p. 461-471, MAR 2019. (18/00432-1, 15/10650-8)

BARBOSA, LILIAN DE MELO; SANTOS, JANDYSON MACHADO; DE MORAIS, DAMILA RODRIGUES; NIMTZ, ALESSANDRA VALERIO; EBERLIN, MARCOS NOGUEIRA; DE OLIVEIRA, MARCELO FIRMINO; COSTA, JOSE LUIZ. Fast UHPLC-MS/MS method for analysis of furanylfentanyl in different seized blotter papers. DRUG TESTING AND ANALYSIS, v. 11, n. 1, p. 178-183, JAN 2019. (18/00432-1, 15/10650-8)