Study of influence of yeast cells treatment on sugarcane ethanol fermentation: Operating conditions and kinetics

Author(s)

Yamakawa, Celina K. ^{[1, 2, 3]} ; Riverab, Elmer Ccopa ^{[2, 3, 4]} ; Kwon, Hyun ^[4] ; Herrera Agudelo, William E. ^{[2, 3]} ; Saad, Marcelo B. W. ^[3] ; Leal, Jairo ^[3] ; Rossell, V, Carlos E. ; Bonomi, Antonio ^{[2, 3]} ; Maciel Filho, Rubens ^{[2, 3]}

Affiliation

^[1] Tech Univ Denmark, Novo Nordisk Fdn Ctr Biosustainabil, Bldg 220, DK-2800 Lyngby - Denmark

^[2] Univ Estadual Campinas, Sch Chem Engn, POB 6066, BR-13081970 Campinas, SP - Brazil

^[3] Brazilian Ctr Res Energy & Mat CTBE CNPEM, Brazilian Bioethanol Sci & Technol Lab, POB 6192, BR-13083970 Campinas, SP - Brazil

^[4] Andrews Univ, Dept Engn & Comp Sci, Berrien Springs, MI 49104 - USA

• Document type: Journal article
• Source: Biochemical Engineering Journal; v. 147, p. 1-10, JUL 15 2019.
• Web of Science Citations: 0

Abstract

In this work, the effects of various operating parameters of the two-stage yeast treatment on the VHG fermentation performance were evaluated. First, a central composite design was used to investigate the effects of temperature and pH on cell viability and flocculation dispersion in the acid treatment unit (1st stage). Second, the effects of temperature, oxidation-reduction potential (ORP) and sugar concentration in the yeast reactivation (2nd stage) on the performance parameters (productivity, ethanol yield and cell viability) of the VHG fermentation were evaluated through a full factorial design. The results indicated that the acid treatment around 2.65 pH and temperature controlled at 30 degrees C for 30 min led to higher cell viability. The conditions of yeast reactivation stage that positively influenced the VHG fermentation performance were relatively low temperature and negative ORP. A mechanistic model quantitatively revealed the influence of the operating parameters studied on cell growth kinetics. The two-stage yeast treatment promoted the increase in cell viability through the cell membrane recovery, which led to the maintenance or improvement the VHG fermentation performance. (AU)

• FAPESP's process: 17/23335-9 - Biorefinery development integrated to a bioethanol sugar cane plant with zero CO2 emission: routes to convert renewable resources to bio-products and bio-electricity
• Grantee: Elmer Alberto Ccopa Rivera
• Support Opportunities: Scholarships in Brazil - Technical Training Program - Technical Training
• FAPESP's process: 11/51902-9 - Simulation of 1st generation sugarcane biorefinery in EMSO platform
• Grantee: Antonio Maria Francisco Luiz Jose Bonomi
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants
• FAPESP's process: 15/20630-4 - Biorefinery development integrated to a bioethanol sugar cane plant with zero CO2 emission: routes to convert renewable resources to bio-products and bio-electricity
• Grantee: Rubens Maciel Filho
• Support Opportunities: Research Projects - Thematic Grants