Low-level laser therapy alleviates the deleterious effect of doxorubicin on rat adipose tissue-derived mesenchymal stem cells

Author(s)

de Lima, Rafael do Nascimento ^[1] ; Vieira, Stella Sousa ^[2] ; Antonio, Ednei Luiz ^[2] ; Camillo de Carvalho, Paulo de Tarso ^[1] ; Vieira, Rodolfo de Paula ^{[3, 4, 5, 6]} ; Dias Martins Mansano, Barbara Sampaio ^[1] ; de Arruda Junior, Daniel Ferreira ^[7] ; Costa Girardi, Adriana Castello ^[7] ; Ferreira Tucci, Paulo Jose ^[2] ; Serra, Andrey Jorge ^{[1, 2]}

Affiliation

^[1] Nove de Julho Univ, Sao Paulo, SP - Brazil

^[2] Univ Fed Sao Paulo, Cardiol Div, Sao Paulo, SP - Brazil

^[3] Brazilian Inst Teaching & Res Pulm & Exercise Imm, Sao Jose Dos Campos, SP - Brazil

^[4] Anhembi Morumbi Univ, Sch Med, Sao Jose Dos Campos, SP - Brazil

^[5] Brasil Univ, Postgrad Program Bioengn & Biomed Engn, Sao Paulo, SP - Brazil

^[6] Univ Fed Sao Paulo, Postgrad Program Sci Human Moviment & Rehabil, Santos, SP - Brazil

^[7] Univ Sao Paulo, Heart Inst, Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 196, JUL 2019.
• Web of Science Citations: 1

Abstract

Cancer is a leading cause of death worldwide, and doxorubicin (DOX) has become one of the most commonly prescribed drugs. Stem cell (SC) therapy is proving to be a promising strategy to alleviate DOX adverse effects on non-cancerous cells. However, the drug also has a toxic action on SCs, reducing the efficiency of cell therapy from a preventive view. The present study shows that the DOX toxicity in mesenchymal SCs (MSCs) can be partially overcome by low-level laser irradiation (LLLI). To achieve this, we applied the low-level red laser (wavelength: 660 nm; output power: 30 mW; laser beam: 0.028 cm(2); irradiation: 1.07 mW/cm(2); Ga-Al-As Photon Laser III, DMC, Sao Paulo, Brazil) in rat adipose tissue-derived MSCs before their exposure to different DOX concentrations. Results revealed that the DOX reduced the viability and adenosine triphosphate level of MSCs. These findings were followed by significantly increased apoptosis as well as oxidative stress in the MSCs. Interestingly, LLLI at the dose of 0.2 J alleviated the effects of DOX on cell viability and apoptosis, and inhibited oxidative stress in the MSCs. In summary, this study provides a crucial step toward the future application of LLLI as a protective approach against DOX-induced toxicity in MSCs, particularly cell death. This study also lays the groundwork for further investigation into the role of oxidative stress and inflammation as an instructive milieu for cell protection. (AU)

• FAPESP's process: 18/06865-7 - Photobiomodulation with light-emitting diode (LED) can improve the efficiency of mesenquimial stem cell therapy in post-ischemic heart failure?
• Grantee: Andrey Jorge Serra
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/14239-0 - Low-intensity laser to modulate the viability, oxidative stress and apoptosis of mesenchymal stem cells from adipose tissue subjected to toxicity of doxorubicin
• Grantee: Rafael Do Nascimento de Lima
• Support Opportunities: Scholarships in Brazil - Master