Novel electrochemical genosensor for Zika virus based on a poly-(3-amino-4-hydroxybenzoic acid)-modified pencil carbon graphite electrode

Author(s)

Alves, Rafael da Fonseca ^[1] ; Franco, Diego Leoni ^[2] ; Cordeiro, Marli Tenorio ^[3] ; de Oliveira Junior, Ernesto Marques ^[3] ; Fireman Dutra, Rosa Amalia ^[4] ; Taboada Sotomayor, Maria Del Pilar ^[1]

Affiliation

^[1] State Univ Sao Paulo UNESP, Dept Analyt Chem, Inst Chem, BR-14801970 Araraquara, SP - Brazil

^[2] Univ Fed Uberlandia, Inst Quim, Grp Eletroquim Aplicada & Polimeros & Sensores, BR-38700128 Patos De Minas, MG - Brazil

^[3] Oswaldo Cruz Fiocruz Fdn, Aggeu Magalhaes Inst, Av Prof Moraes Rego S-N, POB 7-472, BR-50670420 Recife, PE - Brazil

^[4] Univ Fed Pernambuco, Biomed Engn Lab, Av Prof Moraes Rego 1235, BR-50670901 Recife, PE - Brazil

• Document type: Journal article
• Source: SENSORS AND ACTUATORS B-CHEMICAL; v. 296, OCT 1 2019.
• Web of Science Citations: 3

Abstract

The Zika virus has recently emerged as a major worldwide threat because it induces microencephaly and other brain damage in babies born to infected mothers. Epidemiological monitoring of infection has been hampered by the lack of reliable tests capable of distinguishing between Zika and other Flavivirus infections, especially the dengue virus. The present work describes the electrochemical modification of pencil carbon graphite electrodes with a material derived from 3-amino-4-hydroxybenzoic acid. Solutions were prepared in acid medium and the material formed showed insulating behavior. Optical and electrical data suggested that carboxyl groups did not participate in the reaction mechanism. FT-IR spectra indicated that these groups were retained, enabling the proposal of a mechanism based on the data obtained. The material formed was used for immobilization of the ssDNAzv aminated oligonucleotide. Detection of the hybridization was based on the Delta ip resulting from the higher electrostatic repulsion of {[}Fe(CN)(6)](3-), caused by the increase of phosphates by addition of the complementary target sequence. The system showed a good linear correlation coefficient (r(2) = 0.997), with satisfactory selectivity and reproducibility, a low limit of detection (25.4 pM), and precision (RSD) of 3.1%, using real samples of human serum enriched with total extract of Zika virus RNA. Tests of the system using the total RNA extracted from dengue virus types 2 and 3 cultures showed little difference in the observed current, hence confirming the specificity of the system. These findings demonstrated the potential of the proposed biosensor. (AU)

• FAPESP's process: 17/07436-0 - Modification and characterization of low-cost electrodes with material derived from 3-amino-4-hydroxybenzoic acid aiming the development of genosensor applied to the diagnosis of Zika virus
• Grantee: Rafael da Fonseca Alves
• Support Opportunities: Scholarships in Brazil - Master