Genotoxic effects of 4-nonylphenol and Cyproterone Acetate on Rana catesbeiana (anura) tadpoles and juveniles

Author(s)

Gregorio, L. S. ^{[1, 2]} ; Franco-Belussi, L. ^{[3, 4]} ; De Oliveira, C. ^[3]

Affiliation

^[1] Sao Paulo State Univ UNESP, Dept Biol, Grad Program Anim Biol, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil

^[2] UNESP, Letras & Ciencias Exatas, Inst Biociencias, IBILCE, Dept Biol, Rua Cristovao Colombo 2265, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Dept Biol, Sao Jose Do Rio Preto, SP - Brazil

^[4] Fed Univ Mato Grosso Do Sul UFMS, Inst Biosci INBIO, Lab Expt Pathol LAPEx, BR-79002970 Campo Grande, MS - Brazil

• Document type: Journal article
• Source: Environmental Pollution; v. 251, p. 879-884, AUG 2019.
• Web of Science Citations: 0

Abstract

Genotoxic analyses are commonly used in ecotoxicological studies as early biomarkers to investigate the potential effects of environmental contaminants on biological models. Several pollutants can induce DNA damage and, therefore, counting micronuclei and other nuclear abnormalities are efficient tools to evaluate genotoxicity. Some pollutants such as 4-nonylphenol (NP), a detergent used mainly in industries, and Cyproterone Acetate (CPA), an antiandrogenic medicine, have already shown genotoxic effects on some vertebrates. However, although amphibians are considered bioindicators of environmental quality and their populations are declining worldwide, the effects of these compounds on anurans are not yet known and, therefore, we believe that it is important to investigate such effects on anurans. Since water contamination is one of the ultimate causes of amphibian decline, ecotoxicological studies are important to discuss the appropriate solutions to avoid species extinction. Thus, this study investigates the genotoxic effects on Rana catesbeiana tadpoles and juveniles after being exposed to 1,10 and 100 mu g/L NP and 0.025, 0.25 and 2.5 ng/L CPA, by counting the nuclear abnormalities after exposure. The laboratory experiments lasted 28 days. The experimental conditions were the same except for the water volume since tadpoles and juveniles exhibit different habits at different developmental stages. Compared to juveniles, tadpoles were more susceptible to both compounds as indicated by the increased nuclear abnormalities observed in the highest NP concentration and all tested CPA concentrations. The juveniles, on the other hand, responded only to the two highest CPA concentrations. We concluded that CPA, even at very low concentrations, is extremely harmful to both anuran developmental stages and, particularly, to tadpoles. The significant effects observed on tadpoles is an important outcome of this study since 100 mu g/L or higher NP concentrations are frequently detected in the environment. (C) 2019 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 14/00946-4 - Internal Pigmentation in Ectotherms: Innate immunity and UV defense
• Grantee: Lilian Franco Belussi
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 13/02067-5 - Toxic effects of the herbicides Atrazine and Roundup in Eupemphix nattereri (Leiuperidae) and Rhinella schneideri (Bufonidae): morphological changes under different concentrations and abiotic conditions (pH, temperature and photoperiod)
• Grantee: Classius de Oliveira
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/01078-7 - The liver of anuran amphibians: morphology compared between 10 species and action of benzopyrene in the melanomacrophages of Physalaemus cuvieri
• Grantee: Classius de Oliveira
• Support Opportunities: Regular Research Grants