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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Central serotonin prevents hypotension and hypothermia and reduces plasma and spleen cytokine levels during systemic inflammation

Full text
Author(s):
Mota, Clarissa M. D. [1] ; Borges, Gabriela S. [1] ; Amorim, Mateus R. [2] ; Carolino, Ruither O. G. [2] ; Batalhao, Marcelo E. [3] ; Anselmo-Franci, Janete A. [2, 1] ; Carnio, Evelin C. [3] ; Branco, Luiz G. S. [2, 1]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Med Sch Ribeirao Preto, Dept Physiol, Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Dent Sch Ribeirao Preto, Dept Morphol Physiol & Basic Pathol, Ribeirao Preto, SP - Brazil
[3] Univ Sao Paulo, Nursing Sch Ribeirao Preto, Ribeirao Preto, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: BRAIN BEHAVIOR AND IMMUNITY; v. 80, p. 255-265, AUG 2019.
Web of Science Citations: 0
Abstract

An exceptionally high mortality rate is observed in sepsis and septic shock. Systemic administration of lipopolysaccharide (LPS) has been used as an experimental model for sepsis resulting in an exacerbated immune response, brain neurochemistry adjustments, hypotension, and hypothermia followed by fever. Central serotonergic pathways not only modulate systemic inflammation (SI) but also are affected by SI, including in the anteroventral region of the hypothalamus (AVPO), which is the hierarchically most important region for body temperature (Tb) control. In this study, we sought to determine if central serotonin (5-HT) plays a role in SI induced by intravenous administration of LPS (1.5 mg/kg) in male Wistar rats (280-350 g) by assessing 5-HT levels in the AVPO, mean arterial pressure, heart rate, and Tb up to 300 min after LPS administration, as well as assessing plasma and spleen cytokine levels, nitric oxide (NO) plasma levels, and prostaglandin (PG) E-2 levels in the AVPO at 75 min and 300 min after LPS administration. We observed reduced AVPO 5-HT levels, hypotension, tachycardia, hypothermia followed by fever, as well as observing increased plasma NO, plasma and spleen cytokines and AVPO PGE(2) levels in SI. Intracerebroventricular (icy) administration of 5-HT 30 min before LPS administration prevented hypotension and hypothermia, which were accompanied by reduced plasma NO, as well as plasma TNF-alpha, IL-1 beta, IL-6, and IL-10 and spleen TNF-alpha and IL-10 levels. We suggest that SI reduced 5-HT levels in the AVPO favor an increased pro-inflammatory status both centrally and peripherally that converge to hypotension and hypothermia. Moreover, our results are consistent with the notion that exogenous 5-HT given icy prevents hypotension and hypothermia probably activating the splenic anti-inflammatory pathway. (AU)

FAPESP's process: 17/09878-0 - Effect of vagal stimulation during LPS immune challenge in normotensive and spontaneously hypertensive rats
Grantee:Mateus Ramos Amorim
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 16/17681-9 - Pathophysiological changes during systemic inflammation
Grantee:Luiz Guilherme de Siqueira Branco
Support Opportunities: Research Projects - Thematic Grants