MicroRNA expression profiling provides novel insights into immune-related pathways involved in gastric cancer

Author(s)

Martins, Mario Rino ^[1] ; Almeida, Renata Santos ^[2] ; Lucena-Silva, Norma ^[2] ; Coutinho-Camilo, Claudia Malheiros ^[3] ; Torjal, Israel ^[3] ; dos Santos, Rogerio Luiz ^[1] ; Miranda-Furtado, Cristiana Libardi ^[4] ; Lopes Rios, Alvaro Fabricio ^[5] ; Torres, Leuridan Cavalcante ^[6] ; Begnami, Maria Dirlei F. S. ^[7]

Affiliation

^[1] Hosp Canc Pernambuco, Soc Pernambucana Combate Ao Canc, Oncol Surg Dept, Recife, PE - Brazil

^[2] Fundacao Oswaldo Cruz, Aggeu Magalhaes Inst, Recife, PE - Brazil

^[3] AC Camargo Canc Ctr, Int Res Ctr, Sao Paulo - Brazil

^[4] Univ Fed Ceara, Postgrad Program Med & Surg Sci, Drug Res & Dev Ctr, Fortaleza, Ceara - Brazil

^[5] State Univ North Fluminense Darcy Ribeiro, Ctr Biosci & Biotechnol, Rio De Janeiro - Brazil

^[6] Inst Med Integral Prof Fernando Figueira, Translat Res Lab Prof CA Hart, Recife, PE - Brazil

^[7] AC Camargo Canc Ctr, Anat Pathol Dept, Sao Paulo - Brazil

• Document type: Journal article
• Source: MEDICAL ONCOLOGY; v. 36, n. 9 AUG 9 2019.
• Web of Science Citations: 0

Abstract

Gastric cancer is one of the most common cancers, and an increasing number of studies have found that microRNAs (miRNAs) play essential roles in gastric cancer progression; however, the roles of specific miRNAs involved in the immune response to this disease remain unclear. We compared the miRNA expression in tissues from primary gastric cancer patients and healthy controls to find miRNAs dysregulated in gastric cancer and used bioinformatics tools to determine potential roles of these miRNAs in the immune system. We evaluated 25 primary gastric cancer tissues and five healthy gastric tissues. Quantitative real-time polymerase chain reaction was performed for a set of miRNAs, followed by the prediction of their target genes and functional enrichment analysis of these targets. Analysis of a microarray dataset showed that the miRNA miR-196a-5p was significantly upregulated, while miR-374a-5p and miR-375 were downregulated in gastric cancer patients. In addition, miR-374-5p was significantly downregulated in patients with metastasis compared with its expression levels in non-metastatic patients (p = 0.03). Bioinformatics analysis suggested that the pathways regulated by these differentially expressed miRNAs were related to the immune response, cell adhesion, and cell migration. Most importantly, this study provides a new insight into the potential use of multiple miRNAs to find distinct pathways of immune regulation in gastric cancer. (AU)

• FAPESP's process: 12/12551-9 - Determination of miRNA Signature in Gastric Cancer Patients Assigned to Neoadjuvant Chemotherapy.
• Grantee: Michelle Maccarini Barcelos Baldoni
• Support type: Scholarships in Brazil - Doctorate