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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Adverse effects of respiratory disease medicaments and tooth brushing on teeth: A scanning electron microscopy, X-ray fluorescence and infrared spectroscopy study

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Author(s):
Gomes, Raimundo Nonato S. [1, 2] ; Bhattacharjee, Tanmoy T. [3, 4] ; Carvalho, Luis Felipe C. S. [2, 5, 6] ; Soares, Luis Eduardo S. [2]
Total Authors: 4
Affiliation:
[1] Univ Fed Rio de Janeiro, Anna Nery Nursing Sch, Rio De Janeiro - Brazil
[2] Univ Vale Paraiba, Res & Dev Inst, LDAM, Sao Paulo - Brazil
[3] Univ Vale Paraiba, Res & Dev Inst, Lab Nanosensors, Sao Paulo - Brazil
[4] Sir John Walsh Res Ctr, Dunedin - New Zealand
[5] Univ Taubate, Dept Dent, Postgrad Program Dent, Sao Paulo - Brazil
[6] Univ Ctr Braz Cubas, Sao Paulo - Brazil
Total Affiliations: 6
Document type: Journal article
Source: MICROSCOPY RESEARCH AND TECHNIQUE; v. 82, n. 9, p. 1489-1499, SEP 2019.
Web of Science Citations: 0
Abstract

The present study aims to evaluate the effect of brushing with fluoride dentifrice on teeth severely affected by erosion due to respiratory medicaments. Enamel (n = 50) and dentin (n = 50) bovine specimens were prepared and treated with artificial saliva (S-control), acebrofilin hydrochloride (AC), ambroxol hydrochloride (AM), bromhexine hydrochloride (BR), and salbutamol sulfate (SS) and subjected to cycles of demineralization (immersing in 3 mL, 1 min, three times a day at intervals of 1 hr, for 5 days) followed by remineralization (saliva, 37 degrees C, 1 hr). Simulated brushing with fluoridated toothpaste was performed using 810 strokes in a reciprocal-action brushing simulator. Scanning electron microscopy, micro energy dispersive X-ray fluorescence (mu-EDXRF) spectroscopy and attenuated total reflection Fourier transform infrared (ATR FTIR) spectroscopy were then performed. mu-EDXRF images showed extensive erosion after treatment with all medicaments. SEM images showed enamel erosion in order SS > BR > AC = AM > S after brushing and fluoridation. FTIR results were in agreement. In case of dentin, mu-EDXRF measurements showed significant difference in mineral content (percent weight of calcium and phosphate) in SS + brushing + fluoridation treated enamel compared to control, while mu-EDXRF images showed erosive effects in the order SS > AM>BR > AC = S post brushing + fluoridation. SEM images showed erosion in the order SS > AM = BR > AC > S post brushing + fluoridation. Again, FTIR multivariate results were in agreement. Overall, our study shows that proper oral care is critical when taking certain medication. The study also demonstrates the possible use of FTIR for rapid clinical monitoring of tooth erosion in clinics. (AU)