| Full text | |
| Author(s): |
de Santana, Felipe Silva
[1, 2]
;
Gracioso, Louise Hase
[1]
;
Karolski, Bruno
[1]
;
Galluzzi Baltazar, Marcela dos Passos
[3]
;
Mendes, Maria Anita
[3]
;
Oller do Nascimento, Claudio Augusto
[3, 1]
;
Perpetuo, Elen Aquino
[1, 2, 4]
Total Authors: 7
|
| Affiliation: | [1] Univ Sao Paulo, Environm Res & Educ Ctr, CEPEMA, POLI, Conego Domenico Rangoni Rd, 270 Km, Cubatao, SP - Brazil
[2] Univ Sao Paulo, Interunits Grad Program Biotechnol, Sao Paulo - Brazil
[3] Univ Sao Paulo, POLI, Chem Engn Dept, Lineu Prestes Ave 580, Sao Paulo, SP - Brazil
[4] Univ Fed Sao Paulo, Dept Marine Sci, Imar, Unifesp, Carvalho de Mendonca Ave 144, Santos, SP - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Applied Biochemistry and Biotechnology; v. 189, n. 1, p. 103-115, SEP 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
The human exposure to bisphenol A (BPA) occurs frequently. Once, this compound was one of the highest volume chemicals produced worldwide and used as a plasticizer in many products. However, even at low concentration, it can cause severe damage to the endocrine system because of its endocrine disruptor activity. Thus, mitigation studies to remove or reduce this contaminant from the environment are essential. An alternative method of removing it from the environment is the use of bioremediation processes to the selected bacteria isolated from a BPA-impacted area. In this work, four halotolerant strains were isolated from the Santos Estuary System, one of the most important Brazilian examples of environmental degradation. In the present work, one strain presented strong BPA tolerance and high BPA-degrading activity and could grow in a minimum medium containing BPA as the main carbon source. Strain MH137742 was identified as Shewanella haliotis, based on 16S rRNA gene sequencing and mass spectrometry identification by MALDI-TOF Biotyper. Shewanella haliotis was able to tolerate up to 150 mg L-1 of BPA and biotransform 75 mg L-1 in 10 h in a liquid culture medium. Based on the analysis of the produced metabolites by LC-MS, it was possible to predict the metabolic pathway used by this microorganism to degrade the BPA. (AU) | |
| FAPESP's process: | 13/50218-2 - CEPEMA - Cooperative Center of Environmental Engineering |
| Grantee: | Claudio Augusto Oller do Nascimento |
| Support Opportunities: | Research Projects - Thematic Grants |