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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The Xanthomonas citri pv. citri Type VI Secretion System is Induced During Epiphytic Colonization of Citrus

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Author(s):
Ceseti, Lucas M. [1] ; de Santana, Eliane S. [1] ; Ratagami, Camila Y. [1] ; Barreiros, Yasmin [1] ; Lima, Lidia Dos Passos [1] ; Dunger, German [2, 3] ; Farah, Chuck S. [2] ; Alvarez-Martinez, Cristina E. [1]
Total Authors: 8
Affiliation:
[1] Univ Estadual Campinas Unicamp, Dept Genet Evolucao Microbiol & Imunol, Inst Biol, Rua Monteiro Lobato 255, BR-13083862 Campinas, SP - Brazil
[2] Univ Sao Paulo, Dept Bioquim, Inst Quim, Av Prof Lineu Prestes 748, Bloco 0, Sala 10, BR-05508900 Sao Paulo, SP - Brazil
[3] Univ Nacl Litoral, Dept Prod Anim, Fac Ciencias Agr, Kreder 2805, CP 3080HOF, Esperanza, Santa Fe - Argentina
Total Affiliations: 3
Document type: Journal article
Source: Current Microbiology; v. 76, n. 10, p. 1105-1111, OCT 2019.
Web of Science Citations: 0
Abstract

Xanthomonas citri pv. citri (X. citri pv. citri) is the causal agent of Asiatic citrus canker and infects economically important citrus crops. X. citri pv. citri contains one type VI secretion system (T6SS) required for resistance to predation by the soil amoeba Dictyostelium discoideum and induced by the ECF sigma factor EcfK in the presence of amoeba. In this work, we describe the analysis of T6SS gene expression during interaction with host plants. We show that T6SS genes and the cognate positive regulator ecfK are upregulated during growth in the plant surface (epiphytic) and maintain low expression levels during growth inside plant mesophyll. In addition, expression of the virulence-associated T3SS is also induced during epiphytic growth and shows a temporal induction pattern during growth inside plant leaves. The T6SS is not required for adhesion to leaf surface and biofilm formation during the first stages of plant colonization nor for killing of yeasts cells. Since the phyllosphere is colonized by eukaryotic predators of bacteria, induction of the X. citri pv. citri anti-amoeba T6SS during epiphytic growth suggests the presence of an environmental signal that triggers the resistance phenotype. (AU)

FAPESP's process: 11/22571-4 - Regulation of Xanthomonas citri Type IV pilus
Grantee:Ricardo Germán Dunger
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 16/08400-6 - The effects of host plant extracts in Xanthomonas citri subsp citri physiology and protein secretion
Grantee:Camila Yuri Ratagami
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 14/19720-6 - The Xanthomonas citri pv. citri type vi secretion system: function, gene regulation and secreted substrates
Grantee:Cristina Elisa Alvarez Martinez
Support Opportunities: Regular Research Grants
FAPESP's process: 17/02318-9 - Functional studies of the Xanthomonas citri pv. citri type 6 secretion system: secreted proteins, recruitment and regulation
Grantee:Lucas de Moraes Ceseti
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 18/01852-4 - Functional Studies on Xanthomonas citri Type VI Secretion System and Gene Regulation by Alternative Sigma Factors
Grantee:Cristina Elisa Alvarez Martinez
Support Opportunities: Regular Research Grants
FAPESP's process: 14/25261-4 - Gene expression analysis of the Xanthomonas citri subsp citri type VI secretion system
Grantee:Lucas de Moraes Ceseti
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 11/07777-5 - Cyclic di-GMP signaling and the Type IV macromolecule secretion system in Xanthomonas citri
Grantee:Shaker Chuck Farah
Support Opportunities: Research Projects - Thematic Grants