Enzymatic potential and biosurfactant production by endophytic fungi from mangrove forest in Southeastern Brazil

Author(s)

Martinho, Vivian ^[1] ; dos Santos Lima, Lidiane Maria ^[1] ; Barros, Caroline Almeida ^[1] ; Ferrari, Vitor Baptista ^[1] ; Zambrano Passarini, Michel Rodrigo ^[2] ; Santos, Leonardo Andre ^[1] ; de Souza Sebastianes, Fernanda Luisa ^[3] ; Lacava, Paulo Teixeira ^[3] ; de Vasconcellos, Suzan Pantaroto ^[1]

Affiliation

^[1] Fed Univ Sao Paulo UNIFESP, Dept Pharmaceut Sci, R Sao Nicolau 210, BR-09913030 Diadema, SP - Brazil

^[2] Fed Univ Latin Amer Integrat, Latin Amer Inst Life Sci & Nat, Ave Tarquinio Joslin Santos 1000, BR-85870901 Foz do Iguacu, PR - Brazil

^[3] Univ Fed Sao Carlos, Dept Morphol & Pathol, Lab Microbiol & Biomol, Ctr Biol & Hlth Sci, Via Washington Luis Km 235, POB 676, BR-13565905 Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: AMB EXPRESS; v. 9, n. 1 AUG 19 2019.
• Web of Science Citations: 0

Abstract

Microbial activity is the main route for cycling mangrove nutrients. In general, microorganisms have abilities to degrade lignocellulosic compounds. Among the biotechnological potential of the microbiota from mangroves, it is noteworthy about endophytic fungi, which can be considered as effective sources of different bioactive compounds. In this sense, thirty (30) endophytic fungi were isolated from mangrove forest sampling Cananeia, SP, Brazil. These microorganisms were analyzed about their enzymatic activities including: lignin peroxidase EC 1.11.1.14, manganese peroxidase EC 1.11.1.13 and laccase EC 1.10.3.2, as well endo-cellulase EC 3.2.1.4 and endo-xylanase EC 3.2.1.8. Besides that, production of bioactive secondary metabolites like biosurfactant and/or bioemulsifier was also investigated. As results, nineteen (19) isolates were selected about their ligninolytic abilities, nine (9) of them about cellulase activity and thirteen (13) showed xylanase abilities. The fungal isolate named as 3(3), characterized as Fusarium sambucinum, showed a prominent lignin peroxidase (42.4 U L-1) and manganese peroxidase (23.6 U L-1) activities. The isolate 63.1, also related to Fusarium sp. genera, was selected about its laccase activity (41.5 U L-1). From all the investigated fungi, the isolate 47(4) Trichoderma camerunense was selected about its cellulolytic and xylanolytic activities, showing 45.23 and 26.09 U mL(-1), respectively. The same fungi also showed biosurfactant ability demonstrated by superficial tension decreasing to 38 mN/m. In addition, fifteen (15) fungi exhibited bioemulsifier activity, with E-24 values up to 62.8%. (AU)

• FAPESP's process: 04/13910-6 - Biodiversity and functional activities of microorganisms from mangrove of the State of São Paulo
• Grantee: Itamar Soares de Melo
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
• FAPESP's process: 16/23685-7 - Study of the biotransformation of phenylpropanoids dimers by actinomycetes from caatinga
• Grantee: Suzan Pantaroto de Vasconcellos
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants