| Full text | |
| Author(s): Show less - |
Kam, Yiu-Wing
[1]
;
Leite, Juliana Almeida
[2]
;
Amrun, Siti Naqiah
[1]
;
Lum, Fok-Moon
[1]
;
Yee, Wearn-Xin
[1]
;
Abu Bakar, Farhana
[1, 3]
;
Eng, Kai Er
[1]
;
Lye, David C.
[4, 5, 6, 7]
;
Leo, Yee-Sin
[8, 4, 5, 6, 7]
;
Chong, Chia-Yin
[6, 7, 9]
;
Ribas Freitas, Andre Ricardo
[10, 11]
;
Milanez, Guilherme Paier
[2]
;
Proenca-Modena, Jose Luiz
[2]
;
Renia, Laurent
[1]
;
Costa, Fabio T. M.
[2]
;
Ng, Lisa F. P.
[12, 1]
;
Network, Zika-Unicamp
Total Authors: 17
|
| Affiliation: Show less - | [1] ASTAR, Singapore Immunol Network, Singapore 138648 - Singapore
[2] Univ Campinas Unicamp, Dept Genet Evolut Microbiol & Immunol, Inst Biol, Campinas, SP - Brazil
[3] Nanyang Technol Univ, Sch Biol Sci, Singapore - Singapore
[4] Natl Ctr Infect Dis, Singapore - Singapore
[5] Tan Tock Seng Hosp, Singapore - Singapore
[6] Nanyang Technol Univ, Lee Kong Chian Sch Med, Singapore - Singapore
[7] Natl Univ Singapore, Yong Loo Lin Sch Med, Singapore - Singapore
[8] Natl Univ Singapore, Saw Swee Hock Sch Publ Hlth, Singapore - Singapore
[9] KK Womens & Childrens Hosp, Singapore - Singapore
[10] Campinas Dept Publ Hlth Surveillance, Campinas, SP - Brazil
[11] Sao Leopoldo Mandic Med Sch, Campinas, SP - Brazil
[12] Univ Liverpool, Inst Infect & Global Hlth, Liverpool, Merseyside - England
Total Affiliations: 12
|
| Document type: | Journal article |
| Source: | Journal of Infectious Diseases; v. 220, n. 2, p. 203-212, JUL 15 2019. |
| Web of Science Citations: | 1 |
| Abstract | |
Background. Zika virus (ZIKV) infections have reemerged as a global health issue due to serious clinical complications. Development of specific serological assays to detect and differentiate ZIKV from other cocirculating flaviviruses for accurate diagnosis remains a challenge. Methods. We investigated antibody responses in 51 acute ZIKV-infected adult patients from Campinas, Brazil, including 7 pregnant women who later delivered during the study. Using enzyme-linked immunosorbent assays, levels of antibody response were measured and specific epitopes identified. Results. Several antibody-binding hot spots were identified in ZIKV immunogenic antigens, including membrane, envelope (E) and nonstructural protein 1 (NS1). Interestingly, specific epitopes (2 from E and 2 from NS1) strongly recognized by ZIKV-infected patients' antibodies were identified and were not cross-recognized by dengue virus (DENV)-infected patients' antibodies. Corresponding DENV peptides were not strongly recognized by ZIKV-infected patients' antibodies. Notably, ZIKV-infected pregnant women had specific epitope recognition for ZIKV NS1 (amino acid residues 17-34), which could be a potential serological marker for early ZIKV detection. Conclusions. This study identified 6 linear ZIKV-specific epitopes for early detection of ZIKV infections. We observed differential epitope recognition between ZIKV-infected and DENV-infected patients. This information will be useful for developing diagnostic methods that differentiate between closely related flaviviruses. (AU) | |
| FAPESP's process: | 16/00194-8 - Pathogenesis and neurovirulence of emerging viruses in Brazil |
| Grantee: | José Luiz Proença Módena |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 13/25807-4 - Dissecting the mechanisms of infectivity in Plasmodium - Plasmodium yoelii experimental model with implications for the study in Plasmodium vivax. |
| Grantee: | Juliana Almeida Leite |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |