Preparation of advanced hybrid nanomaterials based on functionalized polypyrrole/g...
| Full text | |
| Author(s): |
Mattos, Gabriel J.
[1]
;
Moraes, Jaqueline T.
[1]
;
Barbosa, Eduardo C. M.
[2]
;
Camargo, Pedro H. C.
[2]
;
Dekker, Robert F. H.
[3]
;
Barbosa-Dekker, Aneli M.
[1]
;
Sartori, Elen R.
[1]
Total Authors: 7
|
| Affiliation: | [1] Univ Estadual Londrina, Dept Quim, Ctr Ciencias Exatas, Rodovia Celso Garcia Cid, PR 445, Km 380, BR-86057970 Londrina, PR - Brazil
[2] Univ Sao Paulo, Dept Quim Fundamental, Inst Quim, Av Prof Lineu Prestes 748, BR-05508000 Sao Paulo, SP - Brazil
[3] Univ Tecnol Fed Parana, Programa Posgrad Engn Ambiental, Campus Londrina, BR-86036370 Londrina, PR - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | BIOELECTROCHEMISTRY; v. 129, p. 116-123, OCT 2019. |
| Web of Science Citations: | 1 |
| Abstract | |
In this study, (1 -> 3)(1 -> 6)-beta-D-glucan (botryosphaeran) from Botryosphaeria rhodina MAMB-05 was used, for the first time, to immobilize laccase on a carbon black paste electrode modified with gold nanoparticles. The physicochemical characterization of the proposed laccase-biosensor was performed using transmission electron microscopy and electrochemical impedance spectroscopy. The performance of this novel bio-device was evaluated by choosing hydroquinone as a typical model of a phenolic compound. For hydroquinone determination, experimental variables such as enzyme concentration, pH and operational parameters of the electroanalytical technique were optimized. From square-wave voltammograms, a linear dependence between the cathodic current peak and the hydroquinone concentration was observed within the range 2.00-56.5 mu mol L-1, with a theoretical detection limit of 0.474 mu mol L-1. The proposed method was successfully applied to determine hydroquinone in dermatological cream, and samples from biological and environmental niches. The proposed biosensor device presented good selectivity in the presence of uric acid, various inorganic ions, as well as other phenolic compounds, demonstrating the potential application of this biosensing platform in complex matrices. Operational and analytical stability of the laccase biosensor were evaluated, and demonstrated good intra-day (SD = 0.3%) and inter-day (SD = 3.4%) repeatability and long storage stability (SD = 4.9%). (C) 2019 Elsevier B.V. All rights reserved. (AU) | |
| FAPESP's process: | 15/21366-9 - Hybrid materials containing metal nanoparticles for catalytic applications |
| Grantee: | Pedro Henrique Cury Camargo |
| Support type: | Regular Research Grants |
| FAPESP's process: | 15/26308-7 - Optimization of the physicochemical properties of nano -structured materials for applications in molecular recognition, catalysis and energy conversion/storage |
| Grantee: | Roberto Manuel Torresi |
| Support type: | Research Projects - Thematic Grants |
| FAPESP's process: | 15/11452-5 - Hybrid materials composed of oxides and metallic nanoparticles for catalytic and photocatalytic applications |
| Grantee: | Eduardo César Melo Barbosa |
| Support type: | Scholarships in Brazil - Doctorate (Direct) |