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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Diagnostic fingerprints ISSR/SSR for tropical leguminous species Stylosanthes capitata and Stylosanthes macrocephala

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Author(s):
Ana Lilia Alzate-Marin ; Carolina Costa-Silva ; Priscila Marlys Sá Rivas ; Fernando Bonifacio-Anacleto ; Leticia Gobett Santos ; Rômulo Maciel de Moraes Filho ; Carlos Alberto Martinez
Total Authors: 7
Document type: Journal article
Source: Scientia Agricola; v. 77, n. 3, p. -, 2020.
Web of Science Citations: 2
Abstract

ABSTRACT: Stylosanthes capitata Vogel and Stylosanthes macrocephala M.B.Ferreira & Sousa Costa are two forage leguminous species of agronomic importance for animal husbandry in tropical environments. The physical mixture of both species (80 % S. capitata and 20 % S. macrocephala) comprises the commercial cultivar “Estilosantes Campo Grande”. However, proximity of fields for seed production may contaminate seed lots, compromising seeds quality. The combined use of dominant and co-dominant molecular markers is an appropriate strategy to certificate genetic purity and perform diversity studies of cultivars. In this research, a set of ISSR (Inter-Simple Sequence Repeat polymorphic DNA) and SSR (Simple Sequence Repeat polymorphic DNA) molecular markers were standardized to characterize S. capitata and S. macrocephala species and evaluate the genetic purity of commercial samples. Four ISSR markers (UBC 2, 864, 885, 886) and SSR marker SC18-01 G4B showed precise species-specific electrophoretic fingerprints for both species. Electrophoretic patterns of ISSR molecular markers should be displayed first to confirm the sample identification. The structure analysis showed that the less contaminated sample was S. capitata with 97 % of its genetic composition assigned to a single genetic cluster vs. 95 % for S. macrocephala. S. capitata has greater genetic diversity (ISSRHe:0.292; SSRHe:0.57) than S. macrocephala (ISSRHe:0.285; SSRHe:0.16); however, this difference was only significant with SSR molecular markers. As these genetic resources have considerable ecological, agronomic and economic importance, tools for accurate species identification and genetic studies are essential for further seed multiplication, as well as for improvement and conservation of cultivars. (AU)

FAPESP's process: 14/24817-9 - Analysis of genetic purity of forage legume Stylosanthes capitata Vog. aiming its use in an experiment in TROP-T-FACE system (Temperature Free-air Controlled Enhancement and Free-air Carbon Dioxide Enrichment)
Grantee:Carolina Costa Silva
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 15/23930-9 - CLIMATE WARMING AND HIGH CO2 IMPACT ON GENETIC AND BIOLOGICAL PARAMETERS OF REPRODUCTIVE SYSTEM OF ONE TROPICAL FORAGE LEGUME IN A TROP-T-FACE SIMULATION SYSTEM
Grantee:Ana Lilia Alzate Marin
Support type: Regular Research Grants
FAPESP's process: 08/58075-8 - Miniface climate-change impact experiment to analyze the effects of elevated CO2 and warming on photosynthesis, gene expression, biochemistry, growth, nutrient dynamics and yield of two contrasting tropical forage species
Grantee:Carlos Alberto Martinez y Huaman
Support type: Research Program on Global Climate Change - Thematic Grants