Miniface climate-change impact experiment to analyze the effects of elevated CO2 and warming on photosynthesis, gene expression, biochemistry, growth, nutrient dynamics and yield of two contrasting tropical forage species

• Grant number: 08/58075-8
• Support Opportunities: Research Program on Global Climate Change - Thematic Grants
• Duration: July 01, 2011 - December 31, 2015
• Field of knowledge: Biological Sciences - Botany - Pant Physiology
• Convênio/Acordo: CNPq - Pronex
• Principal Investigator: Carlos Alberto Martinez y Huaman
• Grantee: Carlos Alberto Martinez y Huaman
• Host Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated grant(s): 14/08244-9 - Plant Biology 2014, AR.EXT; 11/21004-9 - Visiting research program of professor Kevin Lee Griffin from the Columbia University to the Department of Biology (thematic project 2008/58075-8) and the Graduate Program in Comparative Biology, FFCLRP/University of São Paulo, AV.EXT; 11/21003-2 - Visiting research program of professor Miquel Gonzales-Meler from the University of Illinois to the Department of Biology (Thematic project 2008/58075-8) and the Graduate Program in Comparative Biology, FFCLRP/University of São Paulo, AV.EXT
• Associated scholarship(s): 14/26821-3 - Comparative analysis of water relations in C3 and C4 forage species subjected to higher CO2 and warming in TROP-T-FACE system, BP.MS; 14/00317-7 - Flux of carbon and greenhouse gases in a pasture ecosystem constituted by Panicum maximum Jacq. and Stylosanthes capitata Voegel cultivated under elevated CO2 and warming in a FACE/T-FACE system, BP.PD; 12/20847-5 - Growth, biomass and efficient use of nutrients in C3 and C4 forages species evaluated by MiniFACE, BP.DR; 12/03439-0 - Effects of elevated CO2 concentration on the dynamics of stable isotopes of carbon and nitrogen in two tropical forage (Panicum maximum and Stylosanthes capitata) cultivated in consortium, BP.MS; 11/04620-8 - Effects of elevated CO2 and warming on physiology of two contrasting tropical forage species, BP.MS

Abstract

The observed and predicted rise in global atmospheric carbon dioxide concentration ([C02]), together with potential global warming and changes in precipitation, will undoubtedly have a significant economical and ecological impact on world agricultural crop plants, grasslands and forests. However, the response of tropical species to elevated [C02] and warming has received little attention to date. The major contribution of this research proposal to FAPESP-RPGCC is regarding the production of new and relevant scientific knowledge with emphasis in adaptation of tropical grazing species to a conservative climate change scenario. This proposal has been specifically designed to address a number of highly focused scientific questions in an interdisciplinary approach (in a national and international collaborative effort among agronomists, biologists, plant physiologists, molecular biologists, husbandry specialists) in order to provide milestones and scientific deliverables regarding the responses of contrasting tropical plants to global climate change. This research proposal aims to determine the effects of elevated CO2 (600 ppm) and warming (+ 2°C), induced by infrared heaters, on the physiological, gene expression, biochemical, growth, carbon and nitrogen dynamics and yield responses of two tropical grazing species Panicum maximum (C4 grass) and Stylosanthes. guianensis (C3 legume) grown in consortium on a climate-change impact experiment using a miniF ACE (Free air CO2 enrichment) system. The hypothesis of the proposal is that exposure of plants to high [C02] impact positively the performance of both functional groups grown in consortium, but the responses of plants will be modified by effect of warning. The major challenge of this project is that it will provide the first study concerning adaptation of grass and leguminous plants growing in consortium to the climate expected 50 years from now and regarding the ability of grazing plants to act as CO2 sink in Brazilian tropics. (AU)

Scientific publications

(36)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ANA LILIA ALZATE-MARIN; CAROLINA COSTA-SILVA; PRISCILA MARLYS SÁ RIVAS; FERNANDO BONIFACIO-ANACLETO; LETICIA GOBETT SANTOS; RÔMULO MACIEL DE MORAES FILHO; CARLOS ALBERTO MARTINEZ. Diagnostic fingerprints ISSR/SSR for tropical leguminous species Stylosanthes capitata and Stylosanthes macrocephala. Scientia Agricola, v. 77, n. 3, 2019-09-05. (08/58075-8, 15/23930-9, 14/24817-9)

HABERMANN, EDUARDO; DIAS DE OLIVEIRA, EDUARDO AUGUSTO; CONTIN, DANIELE RIBEIRO; DELVECCHIO, GUSTAVO; VICIEDO, DILIER OLIVERA; DE MORAES, MARCELA APARECIDA; PRADO, RENATO DE MELLO; DE PINHO COSTA, KATIA APARECIDA; BRAGA, MARCIA REGINA; MARTINEZ, CARLOS ALBERTO. Warming and water deficit impact leaf photosynthesis and decrease forage quality and digestibility of a C4 tropical grass. PHYSIOLOGIA PLANTARUM, v. 165, n. 2, SI, p. 383-402, FEB 2019. (08/58075-8, 14/00317-7, 16/09742-8, 16/12853-6)

VICIEDO, DILIER OLIVERA; PRADO, RENATO DE MELLO; MARTINEZ, CARLOS ALBERTO; HABERMANN, EDUARDO; PICCOLO, MARISA DE CASSIA; HURTADO, ALEXANDER CALERO; BARRETO, RAFAEL FERREIRA; CALZADA, KOLIMA PENA. Changes in soil water availability and air-temperature impact biomass allocation and C:N:P stoichiometry in different organs of Stylosanthes capitata Vogel. Journal of Environmental Management, v. 278, n. 1, JAN 15 2021. (08/58075-8, 16/09742-8)

CARVALHO, JULIANA MARIANO; BARRETO, RAFAEL FERREIRA; PRADO, RENATO DE MELLO; HABERMANN, EDUARDO; FERRAZ BRANCO, ROBERTO BOTELHO; MARTINEZ, CARLOS ALBERTO. Elevated CO2 and warming change the nutrient status and use efficiency of Panicum maximum Jacq. PLoS One, v. 15, n. 3, MAR 13 2020. (14/26821-3, 08/58075-8)

GONZALEZ-MELER, MIQUEL A.; SILVA, LAIS B. C.; DIAS-DE-OLIVEIRA, EDUARDO; FLOWER, CHARLES E.; MARTINEZ, CARLOS A.. Experimental Air Warming of a Stylosanthes capitata, Vogel Dominated Tropical Pasture Affects Soil Respiration and Nitrogen Dynamics. FRONTIERS IN PLANT SCIENCE, v. 8, FEB 1 2017. (08/58075-8, 12/17755-1, 12/03439-0)

DE FREITAS, EMANUELLE N.; KHATRI, VINAY; CONTIN, DANIELE R.; DE OLIVEIRA, TASSIO B.; CONTATO, ALEX G.; PERALTA, ROSANE M.; DOS SANTOS, WANDERLEY D.; MARTINEZ, CARLOS A.; SADDLER, JACK N.; POLIZELI, MARIA DE LOURDES T. M.. limate change affects cell-wall structure and hydrolytic performance of a perennial grass as an energy cro. BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR, v. 16, n. 2, NOV 2021. (08/58075-8, 08/57908-6, 17/23989-9, 19/07141-5, 18/07522-6)

HABERMANN, EDUARDO; DIAS DE OLIVEIRA, EDUARDO AUGUSTO; CONTIN, DANIELE RIBEIRO; DELVECCHIO, GUSTAVO; VICIEDO, DILIER OLIVERA; DE MORAES, MARCELA APARECIDA; PRADO, RENATO DE MELLO; DE PINHO COSTA, KATIA APARECIDA; BRAGA, MARCIA REGINA; MARTINEZ, CARLOS ALBERTO. Warming and water deficit impact leaf photosynthesis and decrease forage quality and digestibility of a C4 tropical grass. PHYSIOLOGIA PLANTARUM, v. 165, n. 2, p. 20-pg., 2019-02-01. (08/58075-8, 16/09742-8, 14/00317-7, 16/12853-6)

ALZATE-MARIN, ANA LILIA; COSTA-SILVA, CAROLINA; SA RIVAS, PRISCILA MARLYS; BONIFACIO-ANACLETO, FERNANDO; SANTOS, LETICIA GOBETT; DE MORAES FILHO, ROMULO MACIEL; MARTINEZ, CARLOS ALBERTO. Diagnostic fingerprints ISSR/SSR for tropical leguminous species Stylosanthes capitata and Stylosanthes macrocephala. CIENTIA AGRICOL, v. 77, n. 3, p. 9-pg., 2020-01-01. (15/23930-9, 08/58075-8, 14/24817-9)

DE FREITAS, EMANUELLE N.; KHATRI, VINAY; WU, JIE; TAKADA, MASATSUGU; SCARCELLA, ANA SILVIA DE A.; MARTINEZ, CARLOS ALBERTO; SADDLER, JACK N.; POLIZELI, MARIA DE LOURDES T. M.. Structural and compositional changes induced by hydrothermal and organosolv pretreatments impacts enzymatic hydrolysis of a tropical forage grass grown under future climate conditions. INDUSTRIAL CROPS AND PRODUCTS, v. 171, NOV 1 2021. (17/23989-9, 18/07522-6, 19/07141-5, 08/57908-6, 08/58075-8)

DE FREITAS, EMANUELLE NEIVERTH; ALNOCH, ROBSON CARLOS; CONTATO, ALEX GRACA; NOGUEIRA, V, KAROLINE MARIA; CREVELIN, EDUARDO JOSE; BERALDO DE MORAES, LUIZ ALBERTO; SILVA, ROBERTO NASCIMENTO; MARTINEZ, CARLOS ALBERTO; POLIZELI, MARIA DE LOURDES T. M.. Enzymatic Pretreatment with Laccases from Lentinus sajor-caju Induces Structural Modification in Lignin and Enhances the Digestibility of Tropical Forage Grass (Panicum maximum) Grown under Future Climate Conditions. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 22, n. 17, SEP 2021. (18/07522-6, 19/07141-5, 08/57908-6, 17/23989-9, 08/58075-8, 20/00081-4)

HABERMANN, EDUARDO; BARRERA SAN MARTIN, JUCA ABRAMO; CONTIN, DANIELE RIBEIRO; BOSSAN, VITOR POTENZA; BARBOZA, ANELIZE; BRAGA, MARCIA REGINA; GROPPO, MILTON; MARTINEZ, CARLOS ALBERTO. Increasing atmospheric CO2 and canopy temperature induces anatomical and physiological changes in leaves of the C-4 forage species Panicum maximum. PLoS One, v. 14, n. 2, FEB 19 2019. (08/58075-8, 14/26821-3)

BRITTO DE ASSIS PRADO, CARLOS HENRIQUE; GUEDES DE CAMARGO-BORTOLIN, LIVIA HAIK; CASTRO, ERIQUE; MARTINEZ, CARLOS ALBERTO. Leaf Dynamics of Panicum maximum under Future Climatic Changes. PLoS One, v. 11, n. 2, FEB 19 2016. (08/58075-8, 12/20847-5)

HABERMANN, EDUARDO; DIAS DE OLIVEIRA, EDUARDO A.; CONTIN, DANIELE RIBEIRO; SAN MARTIN, JUCA A. B.; CURTARELLI, LUCAS; GONZALEZ-MELER, MIQUEL A.; MARTINEZ, CARLOS ALBERTO. Stomatal Development and Conductance of a Tropical Forage Legume Are Regulated by Elevated [CO2] Under Moderate Warming. FRONTIERS IN PLANT SCIENCE, v. 10, MAY 31 2019. (14/26821-3, 14/00317-7, 08/58075-8, 16/09742-8)

HABERMANN, EDUARDO; DE OLIVEIRA, EDUARDO AUGUSTO DIAS; CONTIN, DANIELE RIBEIRO; COSTA, JOAO VITOR CAMPOS PINHO; COSTA, KATIA APARECIDA DE PINHO; MARTINEZ, CARLOS ALBERTO. Warming offsets the benefits of elevated CO2 in water relations while amplifies elevated CO2-induced reduction in forage nutritional value in the C-4 grass Megathyrsus maximus. FRONTIERS IN PLANT SCIENCE, v. 13, p. 15-pg., 2022-12-05. (08/58075-8, 16/09742-8, 14/26821-3, 14/00317-7)

BARRETO, RAFAEL FERREIRA; PRADO, RENATO DE MELLO; HABERMANN, EDUARDO; VICIEDO, DILLER OLIVERA; MARTINEZ, CARLOS ALBERTO. Warming Change Nutritional Status and Improve Stylosanthes capitata Vogel Growth Only Under Well-Watered Conditions. JOURNAL OF SOIL SCIENCE AND PLANT NUTRITION, v. 20, n. 4, p. 10-pg., 2020-05-15. (08/58075-8, 16/09742-8)

APPROBATO, ANDRESSA UEHARA; CONTIN, DANIELE RIBEIRO; OLIVEIRA, EDUARDO AUGUSTO DIAS DE; HABERMANN, EDUARDO; CELA, JANA; PINTO-MARIJUAN, MARTA; MUNNE-BOSCH, SERGI; MARTINEZ, CARLOS ALBERTO. Adjustments in photosynthetic pigments, PS II photochemistry and photoprotection in a tropical C4 forage plant exposed to warming and elevated [CO2]. Plant Physiology and Biochemistry, v. 194, p. 16-pg., 2023-01-01. (08/58075-8, 14/00317-7)

VICIEDO, DILIER OLIVERA; PRADO, RENATO DE MELLO; MARTINEZ, CARLOS A.; HABERMANN, EDUARDO; FERRAZ BRANCO, ROBERTO BOTELHO; PICCOLO, MARISA DE CASSIA; HURTADO, ALEXANDER CALERO; CALZADA, KOLIMA PENA; LATA TENESACA, LUIS F.. Water stress and warming impact nutrient use efficiency of Mombasa grass (Megathyrsus maximus) in tropical conditions. JOURNAL OF AGRONOMY AND CROP SCIENCE, v. 207, n. 1, p. 128-138, FEB 2021. (08/58075-8, 16/09742-8)

BARRETO, RAFAEL FERREIRA; PRADO, RENATO DE MELLO; HABERMANN, EDUARDO; VICIEDO, DILLER OLIVERA; MARTINEZ, CARLOS ALBERTO. Warming Change Nutritional Status and Improve Stylosanthes capitata Vogel Growth Only Under Well-Watered Conditions. JOURNAL OF SOIL SCIENCE AND PLANT NUTRITION, v. 20, n. 4, MAY 2020. (08/58075-8, 16/09742-8)

BORJAS-VENTURA, RICARDO; ALVES, LETICIA R.; DE OLIVEIRA, REGINALDO; MARTINEZ, CARLOS A.; GRATAO, PRISCILA L.. Impacts of warming and water deficit on antioxidant responses in Panicum maximum Jacq. PHYSIOLOGIA PLANTARUM, v. 165, n. 2, SI, p. 413-426, FEB 2019. (08/58075-8)

MARTINEZ, CARLOS ALBERTO; BIANCONI, MATHEUS; SILVA, LAIS; APPROBATO, ANDRESSA; LEMOS, MARIA; SANTOS, LETICIA; CURTARELLI, LUCAS; RODRIGUES, ANDRE; MELLO, TATHYANA; MANCHON, FERNANDA. Moderate warming increases PSII performance, antioxidant scavenging systems and biomass production in Stylosanthes capitata Vogel. ENVIRONMENTAL AND EXPERIMENTAL BOTANY, v. 102, p. 58-67, JUN 2014. (08/58075-8)

DE OLIVEIRA, TASSIO BRITO; DE LUCAS, ROSYMAR COUTINHO; DE ALMEIDA SCARCELLA, ANA SILVIA; CONTATO, ALEX GRACA; PASIN, THIAGO MACHADO; MARTINEZ, CARLOS ALBERTO; TEIXEIRA DE MORAES POLIZELI, MARIA DE LOURDES. Fungal communities differentially respond to warming and drought in tropical grassland soil. Molecular Ecology, v. 29, n. 8, p. 1550-1559, APR 2020. (17/09000-4, 08/58075-8)

DIAS DE OLIVEIRA, EDUARDO AUGUSTO; MANCHON, FERNANDA TOMITA; RICKETTS, MICHAEL P.; BIANCONI, MATHEUS; MARTINEZ, CARLOS ALBERTO; GONZALEZ-MELER, MIQUEL A.. Plant diurnal cycle drives the variation in soil respiration in a C-4-dominated tropical managed grassland exposed to high CO2 and warming. PLANT AND SOIL, v. 456, n. 1-2, p. 391-404, NOV 2020. (08/58075-8, 14/00317-7)

HABERMANN, EDUARDO; CONTIN, DANIELE RIBEIRO; AFONSO, LAURA FERNANDES; BAROSELA, JOSE RICARDO; DE PINHO COSTA, KATIA APARECIDA; VICIEDO, DILIER OLIVERA; GROPPO, MILTON; MARTINEZ, CARLOS ALBERTO. Future warming will change the chemical composition and leaf blade structure of tropical C-3 and C-4 forage species depending on soil moisture levels. Science of The Total Environment, v. 821, p. 13-pg., 2022-02-01. (16/09742-8, 08/58075-8)

OLIVERA VICIEDO, DILIER; PRADO, RENATO DE MELLO; MARTINEZ, CARLOS ALBERTO; HABERMANN, EDUARDO; PICCOLO, MARISA DE CASSIA. Short-term warming and water stress affect Panicum maximum Jacq. stoichiometric homeostasis and biomass production. Science of The Total Environment, v. 681, p. 267-274, SEP 1 2019. (08/58075-8)

WEDOW, JESSICA M.; YENDREK, CRAIG R.; MELLO, TATHYANA R.; CRESTE, SILVANA; MARTINEZ, CARLOS A.; AINSWORTH, ELIZABETH A.. Metabolite and transcript profiling of Guinea grass (Panicum maximum Jacq) response to elevated [CO2] and temperature. METABOLOMICS, v. 15, n. 4, APR 2019. (08/58075-8)

DE CAMARGO-BORTOLIN, L. H. G.; HABERMANN, E.; VICENTE, P.; BARBOZA, A.; GROPPO, M.; PRADO, C. H. B. A.; MARTINEZ, C. A.. Rising CO(2)in the field does not offset warming or drought constraints on leaf growth of a C(3)forage. EXPERIMENTAL AGRICULTURE, v. 56, n. 2, p. 265-279, APR 2020. (12/20847-5, 14/26821-3, 08/58075-8)

GONZALEZ-MELER, MIQUEL A.; RUCKS, JESSICA S.; AUBANELL, GERARD. Mechanistic insights on the responses of plant and ecosystem gas exchange to global environmental change: Lessons from Biosphere. 2. Plant Science, v. 226, n. SI, p. 14-21, SEP 2014. (08/58075-8)

DE OLIVEIRA, TASSIO BRITO; DE LUCAS, ROSYMAR COUTINHO; DE ALMEIDA SCARCELLA, ANA SILVIA; CONTATO, ALEX GRACA; PASIN, THIAGO MACHADO; MARTINEZ, CARLOS ALBERTO; TEIXEIRA DE MORAES POLIZELI, MARIA DE LOURDES. Effects of multiple climate change factors on exoenzyme activities and CO2 efflux in a tropical grassland. SOIL BIOLOGY & BIOCHEMISTRY, v. 148, SEP 2020. (17/09000-4, 08/58075-8)

OLIVERA-VICIEDO, DILIER; PRADO, RENATO DE MELLO; MARTINEZ, CARLOS A.; HABERMANN, EDUARDO; PICCOLO, MARISA DE CASSIA; CALERO-HURTADO, ALEXANDER; BARRETO, RAFAEL FERREIRA; PENA, KOLIMA. Are the interaction effects of warming and drought on nutritional status and biomass production in a tropical forage legume greater than their individual effects?. PLANTA, v. 254, n. 5, NOV 2021. (16/09742-8, 08/58075-8)

HABERMANN, EDUARDO; DIAS DE OLIVEIRA, EDUARDO A.; DELVECCHIO, GUSTAVO; BELISARIO, RAFAEL; BARRETO, RAFAEL FERREIRA; VICIEDO, DILIER OLIVERA; ROSSINGNOLI, NADIA OLIVEIRA; DE PINHO COSTA, KATIA APARECIDA; PRADO, RENATO DE MELLO; GONZALEZ-MELER, MIQUEL; et al. How does leaf physiological acclimation impact forage production and quality of a warmed managed pasture of Stylosanthes capitata under different conditions of soil water availability?. Science of The Total Environment, v. 759, MAR 10 2021. (16/12853-6, 16/09742-8, 08/58075-8)

MALUF, RAQUEL PEREZ; ALZATE-MARIN, ANA LILIA; SILVA, CAROLINA COSTA; PANSARIN, LUDMILA MICKELIUNAS; BONIFACIO-ANACLETO, FERNANDO; SCHUSTER, IVAN; PRADO, RENATO DE MELLO; MARTINEZ, CARLOS A.. Warming and soil water availability affect plant-flower visitor interactions for Stylosanthes capitata, a tropical forage legume. Science of The Total Environment, v. 817, p. 10-pg., 2022-01-15. (08/58075-8)

OLIVERA-VICIEDO, DILIER; OLIVEIRA, KAMILLA SILVA; PRADO, RENATO DE MELLO; HABERMANN, EDUARDO; MARTINEZ, CARLOS ALBERTO; ZANINE, ANDERSON DE MOURA. Silicon uptake and utilization on Panicum maximum grass modifies C:N:P stoichiometry under warming and soil water deficit. SOIL & TILLAGE RESEARCH, v. 235, p. 10-pg., 2024-01-01. (08/58075-8)

BORJAS-VENTURA, RICARDO; ALVES, LETICIA R.; DE OLIVEIRA, REGINALDO; MARTINEZ, CARLOS A.; GRATAO, PRISCILA L.. Impacts of warming and water deficit on antioxidant responses in Panicum maximum Jacq. PHYSIOLOGIA PLANTARUM, v. 165, n. 2, p. 14-pg., 2019-02-01. (08/58075-8)

DE OLIVEIRA, TASSIO BRITO; DE LUCAS, ROSYMAR COUTINHO; DE ALMEIDA SCARCELLA, ANA SILVIA; PASIN, THIAGO MACHADO; MARTINEZ, CARLOS ALBERTO; DE MORAES POLIZELI, MARIA DE LOURDES TEIXEIRA. Perspectives on Exploring Denitrifying Fungi as a Model To Evaluate Nitrous Oxide Production and Reduce Emissions from Agricultural Soils. Journal of Agricultural and Food Chemistry, v. 67, n. 44, p. 2-pg., 2019-11-06. (08/58075-8, 17/09000-4)

VICIEDO, DILIER OLIVERA; PRADO, RENATO DE MELLO; MARTINEZ, CARLOS ALBERTO; HABERMANN, EDUARDO; PICCOLO, MARISA DE CASSIA; HURTADO, ALEXANDER CALERO; BARRETO, RAFAEL FERREIRA; CALZADA, KOLIMA PENA. Changes in soil water availability and air-temperature impact biomass allocation and C:N:P stoichiometry in different organs of Stylosanthes capitata Vogel. Journal of Environmental Management, v. 278, p. 9-pg., 2021-01-15. (08/58075-8, 16/09742-8)

ALZATE-MARIN, ANA LILIA; SA RIVAS, PRISCILA MARLYS; GALASCHI-TEIXEIRA, JULIANA S.; BONIFACIO-ANACLETO, FERNANDO; SILVA, CAROLINA COSTA; SCHUSTER, IVAN; NAZARENO, ALISON GONCALVES; GIULIATTI, SILVANA; DA ROCHA FILHO, LEO CORREIA; GAROFALO, CARLOS A.; et al. Warming and elevated CO2 induces changes in the reproductive dynamics of a tropical plant species. Science of The Total Environment, v. 768, MAY 10 2021. (15/23930-9, 14/24817-9, 08/58075-8)