Shielding and stealth effects of zwitterion moieties in double-functionalized silica nanoparticles

Author(s)

Loiola, Livia M. D. ^[1] ; Batista, Marina ^[1] ; Capeletti, Larissa B. ^{[2, 1]} ; Mondo, Gabriela B. ^{[2, 1]} ; Rosa, Rhubia S. M. ^[3] ; Marques, Rafael E. ^[3] ; Bajgelman, Marcio C. ^[3] ; Cardoso, Mateus B. ^{[2, 1]}

Affiliation

^[1] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Nanotechnol Natl Lab LNNano, BR-13083970 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, UNICAMP, Inst Chem, POB 6154, BR-13083970 Campinas, SP - Brazil

^[3] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Biosci Natl Lab LNBio, BR-13083970 Campinas, SP - Brazil

• Document type: Journal article
• Source: Journal of Colloid and Interface Science; v. 553, p. 540-548, OCT 1 2019.
• Web of Science Citations: 0

Abstract

Surface functionalization of silica nanoparticles (SiO(2)NPs) has been considered as a promising strategy to develop target-specific nanostructures. However, finding a chemical functionalization that can be used as an active targeting moiety while preserving the nanoparticles colloidal stability in biological fluids is still challenging. We present here a dual surface modification strategy for SiO(2)NPs where a zwitterion (ZW) and a biologically active group (BAG) (amino, mercapto or carboxylic functionalities) are simultaneously grafted on the nanoparticles' surface. The rationale behind this strategy is to generate colloidally stable nanoparticles and avoid the nonspecific protein adsorption due to ZW groups insertion, while the effective interaction with biosystems is guaranteed by the BAGs presence. The biological efficacy was tested against VERO cells, E. coli bacteria and Zika viruses and a similar trend was observed for all tested particles. The desirable ``stealth property{''} to prevent nonspecific protein adhesion also generated a ZW shielding effect of the BAG functionality hindering their proper interaction and activity in cells, bacteria and viruses. (C) 2019 Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 17/09203-2 - Surface functionalization of fluorescent silica nanoparticles: a strategy for Zika virus inactivation
• Grantee: Marina Batista
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 15/25406-5 - Organizing matter: colloids formed by association of surfactants, polymers and nanoparticles
• Grantee: Watson Loh
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 16/21598-0 - Functionalized silica nanoparticles: a strategy for Zika virus inactivation
• Grantee: Lívia Mesquita Dias Loiola
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 17/01167-7 - Silica nanoparticles functionalized with carbohydrate oligomers and their interaction with the bacterial membrane
• Grantee: Larissa Brentano Capeletti
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 17/06692-2 - Engineering t cells harboring new transgenic TCRs isolated from tumor infiltrated lymphocytes in animals undergone therapeutic protocols using immunomodulators
• Grantee: Marcio Chaim Bajgelman
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/16905-0 - Colloidal stability and protein corona: fundamental aspects for maintaining silica nanoparticle properties in biologial media
• Grantee: Gabriela Borba Mondo
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)