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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Maternal Protein Restriction Modulates Angiogenesis and AQP9 Expression Leading to a Delay in Postnatal Epididymal Development in Rat

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Author(s):
Santos, Talita de Mello [1] ; Cavariani, Marilia Martins [1] ; Pereira, Dhrielly Natalia [1] ; Schimming, Bruno Cesar [1] ; de Almeida Chuffa, Luiz Gustavo [1] ; Domeniconi, Raquel Fantin [1]
Total Authors: 6
Affiliation:
[1] Sao Paulo State Univ UNESP, Inst Biosci, Dept Anat, BR-18618970 Botucatu, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: CELLS; v. 8, n. 9 SEP 2019.
Web of Science Citations: 0
Abstract

The maternal nutritional status is essential to the health and well-being of the fetus. Maternal protein restriction during the perinatal stage causes sperm alterations in the offspring that are associated with epididymal dysfunctions. Vascular endothelial growth factor (VEGF) and its receptor, VEGFr-2, as well as aquaporins (AQPs) are important regulators of angiogenesis and the epididymal microenvironment and are associated with male fertility. We investigated the effects of maternal protein restriction on epididymal angiogenesis and AQP expression in the early stages of postnatal epididymal development. Pregnant rats were divided into two experimental groups that received either a normoprotein (17% protein) or low-protein diet (6% protein) during gestation and lactation. At postnatal day (PND)7 and PND14, male offspring were euthanized, the epididymides were subjected to morphometric and microvascular density analyses and to VEGF-A, VEGF-r2, AQP1 and AQP9 expression analyses. The maternal low-protein diet decreased AQP9 and VEGFr-2 expression, decreased epididymal microvascularity and altered the morphometric features of the epididymal epithelium; no changes in AQP1 expression were observed at the beginning of postnatal epididymal development. Maternal protein restriction alters microvascularization and affects molecules involved in the epidydimal microenvironment, resulting in morphometric alterations related to a delay in the beginning of epididymis postnatal development. (AU)

FAPESP's process: 16/04085-9 - Wolffian duct development, differentiation and vascular pattern of epididymis in Wistar rats exposed to protein restriction during pregnancy and lactation
Grantee:Raquel Fantin Domeniconi
Support Opportunities: Regular Research Grants
FAPESP's process: 17/23953-4 - Epididymis growth factors analysis in rats whose mothers were submitted to protein restriction during gestation and lactation
Grantee:Dhrielly Natalia Pereira
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 15/18767-1 - Effects of maternal protein restriction on the vascular pattern and protein expression in male Wistar rat epididymis at different stages of postnatal development
Grantee:Marilia Martins Cavariani
Support Opportunities: Scholarships in Brazil - Doctorate