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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Alternative biodegradation pathway of the herbicide diuron

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Author(s):
Silva Moretto, Jessica Aparecida [1] ; Rueda Furlan, Joao Pedro [1] ; Tonelli Fernandes, Ana Flavia [1] ; Bauermeister, Anelize [2] ; Lopes, Norberto Peporine [2] ; Stehling, Eliana Guedes [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Fac Ciencias Fannaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, Ribeirao Preto - Brazil
[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, NPPNS, Ribeirao Preto, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: INTERNATIONAL BIODETERIORATION & BIODEGRADATION; v. 143, SEP 2019.
Web of Science Citations: 1
Abstract

Native soil microbiota is important to maintain the quality of the environment, but with the intensive use of agrochemicals, changes in microbial biomass and formation of toxic waste have been observed. Studies have emphasized the importance of using bioremediation to reduce the diuron in the environment, as well as other pesticides. The biodegradation of diuron is usually described under aerobic conditions, in which microorganisms perform an N-demethylation of the urea group and subsequent hydrolysis, producing three different metabolites: 3-(3,4-dichlorophenyl)-3-methylurea, 3,4-dichlorophenylurea, and 3,4-dichloroaniline. In this study, the biodegradation of this herbicide by bacteria isolated from a sugarcane cultivation area and the degradation pathway of diuron were evaluated. The metabolites generated from diuron biodegradation were analyzed by High Performance Liquid Chromatography coupled to a mass spectrometer with an electrospray ionization source and ion trap analyzer. Isolate 5204, identified as Escherichia fergusonii by sequencing of the 16S rDNA, adk, gyrB and recA was able to remove the chlorine from the herbicide molecule, as well as forming the metabolites already described. Therefore, these results suggest that isolate 5204 can degrade diuron by a different pathway not yet described. (AU)

FAPESP's process: 15/18990-2 - Investigation of quinolone resistance mechanisms in environmental bacterial isolates
Grantee:Eliana Guedes Stehling
Support Opportunities: Regular Research Grants