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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Laser Photobiomodulation 904nm Promotes Inhibition of Hormone-Sensitive Lipase Activity in 3T3-L1 Adipocytes Differentiated Cells

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Author(s):
Mafra, Fernando F. P. [1] ; Macedo, Michel M. [1] ; Orphao, Juliana do N. P. [1] ; Lopes, Arthur Vecchi [1] ; Teixeira, Carla de Brito [1] ; Gattai, Pedro P. [2] ; Torres-Silva, Romildo [1] ; Nascimento, Fabio Dupart [1] ; Brandao Lopes-Martins, Rodrigo Alvaro [3]
Total Authors: 9
Affiliation:
[1] Univ Mogi das Cruzes, Technol Res Ctr, Mogi das Cruzes - Brazil
[2] Fed Univ Sa Paulo UNIFESP, Mol Biol Lab, Div Renal, Dept Med, Mol - Brazil
[3] Univ Vale Paraiba Univ, Inst Res & Dev, Ave Shishima Hifumi 2911, BR-12244000 Sao Jose Dos Campos, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: PHOTOBIOMODULATION PHOTOMEDICINE AND LASER SURGERY; v. 37, n. 2, p. 66-69, FEB 2019.
Web of Science Citations: 0
Abstract

Background: The lipid metabolism is essential for maintaining the body's energy responses. Laser photobiomodulation triggers many important cellular effects, but these effects on lipid metabolism are not well described. In this study, we analyzed the laser photobiomodulation in the hormone-sensitive lipase (HSL) activity, a key enzyme in the triglycerides (TAG) hydrolysis in adipose tissue 3T3-L1. Methods: Cells were submitted to the differentiation protocol in adipose cells, irradiated with 1, 2, and 3J with laser (904 nm-60mw-laser diode) and incubated for 4 h after irradiation. Results: The response of laser photobiomodulation was able to trigger an inhibition of HSL activity (control = 0.057 - 0.0008; 1J = 0.050 +/- 0.0003; 2J = 0.0477 +/- 0.002; 3J = 0.051 +/- 0.002; p = 0.0003 against the control), but no modulation was observed in TAG levels into the medium (control = 26.5856 +/- 0.52; 1J = 26.5856 +/- 0.52; 2J = 27.2372 +/- 1.41; 3J = 25.9991 +/- 0.1303; p = 0.18). Conclusions: This is the first study of HSL activity modulation with laser radiation, suggesting that photobiomodulation can influence adipose tissue metabolism and open a new field of study. (AU)

FAPESP's process: 15/06502-3 - Analysis and Modelling of Mechanical Behavior of Mechanicaly-lesioned Tendons treated with Low Level Laser Therapy and/or Cryotherapy
Grantee:Rodrigo Alvaro Brandão Lopes Martins
Support Opportunities: Regular Research Grants