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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Antifungal compounds from Streptomyces associated with attine ants also inhibit Leishmania donovani

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Author(s):
Ortega, Humberto E. [1] ; Ferreira, Leonardo L. G. [2] ; Melo, Weilan G. P. [1] ; Oliveira, Ana Ligia L. [3, 1] ; Ramos Alvarenga, Rene F. [4] ; Lopes, Norberto P. [1] ; Bugni, Tim S. [4] ; Andricopulo, Adriano D. [2] ; Pupo, Monica T. [1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Inst Fis Sao Carlos, Lab Quim Med & Computac, Ctr Pesquisa & Inovacao Biodiversidade & Farmacos, Sao Carlos, SP - Brazil
[3] Univ Fed Amazonas, Fac Ciencias Farmaceut, Manaus, Amazonas - Brazil
[4] Univ Wisconsin Madison, Div Pharmaceut Sci, Madison, WI - USA
Total Affiliations: 4
Document type: Journal article
Source: PLoS Neglected Tropical Diseases; v. 13, n. 8 AUG 2019.
Web of Science Citations: 0
Abstract

Bacterial strains isolated from attine ants showed activity against the insect specialized fungal pathogen Escovopsis and also against the human protozoan parasite Leishmania donovani. The bioassay guided fractionation of extracts from cultures of Streptomyces sp. ICBG292, isolated from the exoskeleton of Cyphomyrmex workers, led to the isolation of Mer-A2026B (1), piericidin-A(1) (2) and nigericin (3). Nigericin (3) presented high activity against intracellular amastigotes of L. donovani (IC50 0.129 +/- 0.008 mu M). Streptomyces puniceus ICBG378, isolated from workers of Acromyrmex rugosus rugosus, produced dinactin (4) with potent anti-L. donovani activity against intracellular amastigotes (IC50 0.018 +/- 0.003 mu M). Compounds 3 and 4 showed good selectivity indexes, 88.91 and 656.11 respectively, and were more active than positive control, miltefosine. Compounds 1-4 were also active against some Escovopsis strains. Compounds 1 and 2 were also produced by Streptomyces sp. ICBG233, isolated from workers of Atta sexdens, and detected in ants' extracts by mass spectrometry, suggesting they are produced in the natural environment as defensive compounds involved in the symbiotic interaction. (AU)

FAPESP's process: 13/07600-3 - CIBFar - Center for Innovation in Biodiversity and Drug Discovery
Grantee:Glaucius Oliva
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 13/50954-0 - Novel therapeutic agents from the bacterial symbionts of Brazilian invertebrates
Grantee:Mônica Tallarico Pupo
Support type: BIOTA-FAPESP Program - Thematic Grants
FAPESP's process: 15/01001-6 - Discovering bacterial symbionts diversity associated with ants in different Brazilian biomes
Grantee:Weilan Gomes da Paixão Melo
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 14/14095-6 - Bacterial symbionts isolated from fungus-growing ants collected in remaining hotspots of biodiversity in São Paulo State as sources of antiparasitic compounds
Grantee:Humberto Enrique Ortega Dominguez
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 16/15872-1 - Identification of natural antiparasitic products produced by insect–bacterial symbiosis
Grantee:Ana Lígia Leandrini de Oliveira
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 16/20154-0 - Exploring antimicrobial, anticancer and antiparasitic compounds from bacterial symbionts isolated from Acromyrmex ants, collected in São Paulo State, Brazil
Grantee:Humberto Enrique Ortega Dominguez
Support type: Scholarships abroad - Research Internship - Doctorate (Direct)