| Full text | |
| Author(s): Show less - |
Sgrignoli, Marcos Rogerio
[1]
;
Silva, Danielle Alves
[1]
;
Nascimento, Felipe Franco
[1]
;
Motta Sgrignoli, Danielle Antonelli
[1]
;
Nai, Gisele Alborghetti
[1, 2]
;
da Silva, Marcia Guimaraes
[3]
;
de Barros, Michele Andrade
[4]
;
Krahembuhl, Maura
[4]
;
Bittencourt, Wanderley
[4]
;
de Morais, Bruna Pereira
[5]
;
Dinallo, Heloise Rangel
[5]
;
Duran Foglia, Bruna Toledo
[5]
;
Cabrera, Wellington Bott
[5]
;
Fares, Elaine Carrion
[5]
;
Andrade, Silvia Franco
[1]
Total Authors: 15
|
| Affiliation: | [1] Univ Oeste Paulista UNOESTE, Postgrad Program Anim Sci, Presidente Prudente, SP - Brazil
[2] Univ Oeste Paulista, Dept Pathol, Fac Med, Presidente Prudente, SP - Brazil
[3] UNESP, Dept Pathol, Fac Med, Botucatu, SP - Brazil
[4] Regenera Stem Cell Lab, Campinas, SP - Brazil
[5] Univ Oeste Paulista, Fac Vet Med, Presidente Prudente, SP - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | STEM CELL RESEARCH; v. 39, AUG 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
Keratoconjunctivitis sicca (KCS) is of predominantly immune-mediated origin. Dogs are an excellent model for understanding this disease, as the origin of KCS in dogs is like that in humans. The objective of this study was to localize and quantify immunological markers, such as CD4 lymphocytes, interleukin (IL)-1, IL-6 and tumor necrosis factor alpha (TNF alpha), before and after topical treatment with mesenchymal stem cells (MSCs). Twenty-two dogs positive for KCS were topically treated with 50 mu L (1 x 10(6) MSCs) in the conjunctival sac and were evaluated for 6 months. The levels of the markers CD4, IL-6, IL-1 and TNF alpha were analyzed in conjunctival biopsy and cytology of the third eyelid gland by immunohistochemistry and immunocytochemistry. The results showed that before treatment, there was marked expression of all the markers (CD4, IL-6, IL-1 and TNF alpha), and after 6 months, there were significant (p < .05) reductions in the expression levels of all the markers. These results demonstrated that topical MSC treatment promotes a significant decrease in the expression levels of these inflammatory markers and could be used as adjuvant therapy in the treatment of KCS in dogs and humans. In addition, these markers can be excellent tools for diagnosing and analyzing the progression of KCS. (AU) | |
| FAPESP's process: | 16/07044-1 - Topical use of heterologous mesenchymal stem cells from canine adipose tissue in the treatment of keratoconjunctivitis sicca in dogs |
| Grantee: | Silvia Maria Caldeira Franco Andrade |
| Support Opportunities: | Regular Research Grants |