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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Dietary sulfur amino acid restriction upregulates DICER to confer beneficial effects

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Author(s):
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Guerra, Beatriz A. [1, 2, 3] ; Brandao, Bruna B. [1, 3] ; Pinto, Silas S. [1, 2, 4, 3] ; Salgueiro, Willian G. [2, 4] ; De-Souza, Evandro A. [1, 5, 3] ; Reis, Felipe C. G. [1] ; Batista, Thiago M. [6] ; Cavalcante-Silva, Vanessa [7] ; D'Almeida, Vania [7] ; Castilho, Beatriz A. [8] ; Carneiro, Everardo M. [6] ; Antebi, Adam [9, 10] ; Festuccia, William T. [11] ; Mori, Marcelo A. [1, 2, 4, 3]
Total Authors: 14
Affiliation:
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[1] Univ Fed Sao Paulo, Escola Paulista Med, Dept Biophys, Sao Paulo - Brazil
[2] Univ Estadual Campinas, Inst Biol, Dept Biochem & Tissue Biol, Campinas, SP - Brazil
[3] Univ Fed Sao Paulo, Escola Paulista Med, Program Mol Biol, Sao Paulo - Brazil
[4] Univ Estadual Campinas, Inst Biol, Program Genet & Mol Biol, Campinas, SP - Brazil
[5] Univ Fed Rio de Janeiro, Inst Med Biochem Leopoldo de Meis, Rio De Janeiro - Brazil
[6] Univ Estadual Campinas, Inst Biol, Dept Struct & Funct Biol, Campinas, SP - Brazil
[7] Univ Fed Sao Paulo, Escola Paulista Med, Dept Psychobiol, Sao Paulo - Brazil
[8] Univ Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, Sao Paulo - Brazil
[9] Max Planck Inst Biol Ageing, Cologne - Germany
[10] Univ Cologne, CECAD, Cologne - Germany
[11] Univ Sao Paulo, Inst Ciencias Biomed, Dept Physiol, Sao Paulo - Brazil
Total Affiliations: 11
Document type: Journal article
Source: MOLECULAR METABOLISM; v. 29, p. 124-135, NOV 2019.
Web of Science Citations: 0
Abstract

Objective: Dietary restriction (DR) improves health and prolongs lifespan in part by upregulating type III endoribonuclease DICER in adipose tissue. In this study, we aimed to specifically test which missing dietary component was responsible for DICER upregulation. Methods: We performed a nutrient screen in mouse preadipocytes and validated the results in vivo using different kinds of dietary interventions in wild type or genetically modified mice and worms, also testing the requirement of DICER on the effects of the diets. Results: We found that sulfur amino acid restriction (i.e., methionine or cysteine) is sufficient to increase Dicer mRNA expression in preadipocytes. Consistently, while DR increases DICER expression in adipose tissue of mice, this effect is blunted by supplementation of the diet with methionine, cysteine, or casein, but not with a lipid or carbohydrate source. Accordingly, dietary methionine or protein restriction mirrors the effects of DR. These changes are associated with alterations in serum adiponectin. We also found that DICER controls and is controlled by adiponectin. In mice, DICER plays a role in methionine restriction-induced upregulation of Ucpl in adipose tissue. In C. elegans, DR and a model of methionine restriction also promote DICER expression in the intestine (an analog of the adipose tissue) and prolong lifespan in a DICER-dependent manner. Conclusions: We propose an evolutionary conserved mechanism in which dietary sulfur amino acid restriction upregulates DICER levels in adipose tissue leading to beneficial health effects. (C) 2019 The Authors. Published by Elsevier GmbH. (AU)

FAPESP's process: 15/03292-8 - Study of the role of GCN1 in the genesis of metabolic diseases in mice
Grantee:Beatriz Alves Guerra
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 17/22057-5 - Physiological effects and transgenerational inheritance induced by cholesterol-rich diet in Caenorhabditis elegans
Grantee:Willian Goulart Salgueiro
Support type: Scholarships in Brazil - Master
FAPESP's process: 16/02207-0 - Identification of soma-germline interactions that may influence lifespan in Caenorhabditis elegans
Grantee:Evandro Araújo de Souza
Support type: Scholarships abroad - Research Internship - Master's degree
FAPESP's process: 17/01184-9 - CAMeLEOm: cross-species analysis of metabolic, lifespan effects and omics of dietary restriction mimetics
Grantee:Marcelo Alves da Silva Mori
Support type: Research Projects - Thematic Grants
FAPESP's process: 15/01316-7 - Dicer, miRNAs and the control of mitochondrial function in the context of aging and caloric restriction
Grantee:Marcelo Alves da Silva Mori
Support type: Regular Research Grants
FAPESP's process: 15/19530-5 - Involvement of the nutrient sensor mTOR in the development of obesity associated chronic metabolic diseases
Grantee:William Tadeu Lara Festuccia
Support type: Research Projects - Thematic Grants
FAPESP's process: 12/07259-7 - Evaluation of miRNA processing in adipose tissue of mice subjected to caloric restriction
Grantee:Beatriz Alves Guerra
Support type: Scholarships in Brazil - Master
FAPESP's process: 17/07975-8 - Exploring the role of adipose tissue-derived circulating miRNAs in the beneficial effects of exercise training
Grantee:Marcelo Alves da Silva Mori
Support type: Regular Research Grants