Effects of nitric oxide-releasing nanoparticles on neotropical tree seedlings submitted to acclimation under full sun in the nursery

Author(s)

Lopes-Oliveira, Patricia Juliana ^[1] ; Gomes, Diego Genuario ^[1] ; Pelegrino, Milena Trevisan ^[2] ; Bianchini, Edmilson ^[1] ; Pimenta, Jose Antonio ^[1] ; Stolf-Moreira, Renata ^[1] ; Seabra, Amedea Barozzi ^[2] ; Oliveira, Halley Caixeta ^[1]

Affiliation

^[1] Univ Estadual Londrina, Dept Anim & Plant Biol, Km 380, BR-86057970 Londrina, PR - Brazil

^[2] Univ Fed ABC UFABC, Ctr Nat & Human Sci, Av Estados 5001, BR-09210580 Santo Andre, SP - Brazil

• Document type: Journal article
• Source: SCIENTIFIC REPORTS; v. 9, NOV 22 2019.
• Web of Science Citations: 0

Abstract

Polymeric nanoparticles have emerged as carrier systems for molecules that release nitric oxide (NO), a free radical involved in plant stress responses. However, to date, nanoencapsulated NO donors have not been applied to plants under realistic field conditions. Here, we verified the effects of free and nanoencapsulated NO donor, S-nitroso-mercaptosuccinic acid (S-nitroso-MSA), on growth, physiological and biochemical parameters of neotropical tree seedlings kept under full sunlight in the nursery for acclimation. S-nitroso-MSA incorporation into chitosan nanoparticles partially protected the NO donor from thermal and photochemical degradation. The application of nanoencapsulated S-nitroso-MSA in the substrate favoured the growth of seedlings of Heliocarpus popayanensis, a shade-intolerant tree. In contrast, free S-nitroso-MSA or nanoparticles containing non-nitrosated mercaptosuccinic acid reduced photosynthesis and seedling growth. Seedlings of Cariniana estrellensis, a shade-tolerant tree, did not have their photosynthesis and growth affected by any formulations, despite the increase of foliar S-nitrosothiol levels mainly induced by S-nitroso-MSA-loaded nanoparticles. These results suggest that depending on the tree species, nanoencapsulated NO donors can be used to improve seedling acclimation in the nursery. (AU)

• FAPESP's process: 18/08194-2 - Essential oil amended with metal nanoparticles functionalized with nitric oxide as a strategy to control plant pathogens in the agriculture
• Grantee: Amedea Barozzi Seabra
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/05029-8 - Preparation, characterization and biological evaluation of nitric oxide releasing polymeric biomaterials
• Grantee: Milena Trevisan Pelegrino
• Support Opportunities: Scholarships in Brazil - Doctorate