Advanced search
Start date
Betweenand
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

In Vitro Schistosomicidal Activity of the Alkaloid-Rich Fraction from Ruta graveolens L. (Rutaceae) and Its Characterization by UPLC-QTOF-MS

Full text
Author(s):
Aleixo de Carvalho, Lara Soares [1] ; Queiroz, Lucas Sales [1] ; Alves Junior, Ismael Jose [1] ; Almeida, Ayla das Chagas [2] ; Coimbra, Elaine Soares [2] ; Pinto, Priscila de Faria [3] ; Nascimento da Silva, Marcos Paulo [4] ; De Moraes, Josue [4] ; Da Silva Filho, Ademar A. [1]
Total Authors: 9
Affiliation:
[1] Univ Fed Juiz de Fora, Fac Pharm, Dept Pharmaceut Sci, BR-36036900 Juiz De Fora, MG - Brazil
[2] Univ Fed Juiz de Fora, Biol Sci Inst, Dept Parasitol Microbiol & Immunol, BR-36036900 Juiz De Fora, MG - Brazil
[3] Univ Fed Juiz de Fora, Biol Sci Inst, Dept Biochem, BR-36036900 Juiz De Fora, MG - Brazil
[4] Univ Guarulhos, Nucleo Pesquisa Doencas Negligenciadas, BR-07025000 Guarulhos, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Evidence-based Complementary and Alternative Medicine; v. 2019, NOV 16 2019.
Web of Science Citations: 0
Abstract

Schistosomiasis is a neglected tropical disease that affects million people worldwide, mostly in developing countries. Ruta graveolens (Rutaceae) is a plant used in folk medicine to treat several diseases, including parasitic infections. In this study, we reported the in vitro schistosomicidal activity of the R. graveolens extract (Rg) and its active fraction (Rg-FAE). Also, the characterization of Rg-FAE by UPLC-ESI-QTOF-MS analysis and its in vitro antileishmanial activity against Leishmania braziliensis were also performed. In vitro schistosomicidal assays were assessed against adult worms of S. mansoni, while cell viability against peritoneal macrophages was measured by MTT assay. Rg (100 mu g/mL) exhibited noticeable schistosomicidal activity, causing 100% mortality and decreasing motor activity of all adult male and female schistosomes, but with low activity against L. braziliensis. After chromatographic fractionation of Rg, fraction Rg-FAE was obtained, showing high activity against adult schistosomes. UPLC-ESI-QTOF-MS analysis of Rg-FAE revealed the presence of eleven alkaloids and one furanocoumarin. No significant antileishmanial activity was found for Rg, while Rg-FAE exhibited activity against L. braziliensis promastigotes. We demonstrated, for the first time, that the R. graveolens extract (Rg) and its alkaloid-rich fraction (Rg-FAE) are active against adult worms of S. mansoni, with no significant cytotoxicity on macrophages. Our findings open the route to further antiparasitic studies with the active fraction of R. graveolens and its identified compounds, especially alkaloids. (AU)

FAPESP's process: 16/22488-3 - Drug repositioning for neglected diseases: identification of novel anthelmintic agents
Grantee:Josué de Moraes
Support Opportunities: Regular Research Grants