Development and evaluation of sun protection factor of emulsion containing extract rich in flavonoids

• Grant number: 17/14757-7
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: October 23, 2017
• End date: February 22, 2018
• Field of knowledge: Health Sciences - Pharmacy
• Principal Investigator: Priscila Gava Mazzola
• Grantee: Leticia Caramori Cefali
• Supervisor: Eliana Maria Barbosa Souto
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Institution abroad: Universidade de Coimbra (UC), Portugal
• Associated to the scholarship: 15/25533-7 - DEVELOPMENT AND EVALUATION OF A FLAVONOID ENRICHED EMULSION WITH SUN PROTECTION FACTOR, BP.DR

Abstract

Sunscreen usage is necessary to avoid skin diseases, specially skin cancer, besides preventing premature skin aging. The aim of this study is to evaluate the potential of sun protection factor of extract containing flavonoids in order to be incorporated in topical formulation. For this, four vegetal species (Dirmophandra mollis Benth, Ginkgo biloba L., Ruta graveolens L. and Vitis vinífera L.) were used at work to obtain extract rich in flavonoids. The extracts obtained for each species and the extract containing all of them, named Mix, were submitted to the following assays: flavonoids identification; rutin and quercetin quantification by high performance liquid chromatography; in vitro citotoxity; sun protect factor and antioxidant efficacy, both in vitro. After to realize these assays, nine emulsion (O/W) were development and they were submitted physic-chemical stability study. The most stables formulations were sensory evaluated by volunteers and the favorite was used as vehicle to formula containing extract. Formulation containing extract has been evaluated for physic-chemical stability and spreadability, rheology, antioxidant activity and sun protect factor, both in vitro. Posteriorly thermal analysis and cutaneous permeation and retention tests, using Franz cells, were realized. Partial results showed that extracts presented flavonoids referents to quercetin and rutin, are secure for citotoxicy assay realized, presented sun protection factor and antioxidant activity, according methods realized, and Ginkgo biloba L. extract and Mix sample presented the most significant results. The cosmetic formulation development and chosen such as favorite by sensory analysis presented physic-chemical stability and good spreadability, and formulation containing flavonoids presented antioxidant activity and sun protection factor by in vitro methods. Therefore, after finish of study, formula containing flavonoids will be an alternative to combat the deleterious effects from exposure to ultraviolet radiation and a promise to use as sunscreen.

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CEFALI, LETICIA CARAMORI; ATAIDE, JANAINA ARTEM; FERNANDES, ANA RITA; DE OLIVEIRA SOUSA, ILZA MARIA; DA SILVA GONCALVES, FERNANDA CRISTINA; EBERLIN, SAMARA; DAVILA, JOSE LUIS; JOZALA, ANGELA FAUSTINO; CHAUD, MARCO VINICIUS; SANCHEZ-LOPEZ, ELENA; et al. Flavonoid-Enriched Plant-Extract-Loaded Emulsion: A Novel Phytocosmetic Sunscreen Formulation with Antioxidant Properties. ANTIOXIDANTS, v. 8, n. 10, OCT 2019. (15/25533-7, 17/14757-7)

CEFALI, LETICIA CARAMORI; ATAIDE, JANAINA ARTEM; FERNANDES, ANA RITA; SANCHEZ-LOPEZ, ELENA; DE OLIVEIRA SOUSA, ILZA MARIA; FIGUEIREDO, MARIANA CECCHETTO; TASCA GOIS RUIZ, ANA LUCIA; FOGLIO, MARY ANN; MAZZOLA, PRISCILA GAVA; SOUTO, ELIANA BARBOSA. Evaluation of In Vitro Solar Protection Factor (SPF), Antioxidant Activity, and Cell Viability of Mixed Vegetable Extracts from Dirmophandra mollis Benth, Ginkgo biloba L., Ruta graveolens L., and Vitis vinifera L.. PLANTS-BASEL, v. 8, n. 11, NOV 2019. (17/14757-7, 15/25533-7)

FRANCO, JULIA GOMES; CEFALI, LETICIA CARAMORI; ATAIDE, JANAINA ARTEM; SANTINI, ANTONELLO; SOUTO, ELIANA B.; MAZZOLA, PRISCILA GAVA. Effect of nanoencapsulation of blueberry (Vaccinium myrtillus): A green source of flavonoids with antioxidant and photoprotective properties. SUSTAINABLE CHEMISTRY AND PHARMACY, v. 23, OCT 2021. (15/25533-7, 17/14757-7, 17/05275-9)

CEFALI, LETICIA CARAMORI; ATAIDE, JANAINA ARTEM; EBERLIN, SAMARA; DA SILVA GONCALVES, FERNANDA CRISTINA; FERNANDES, ANA RITA; MARTO, JOANA; RIBEIRO, HELENA MARGARIDA; FOGLIO, MARY ANN; MAZZOLA, PRISCILA GAVA; SOUTO, ELIANA BARBOSA. In vitro SPF and Photostability Assays of Emulsion Containing Nanoparticles with Vegetable Extracts Rich in Flavonoids. AAPS PHARMSCITECH, v. 20, n. 1, JAN 2019. (15/25533-7, 17/14757-7)