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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Determination of antimicrobial susceptibility and biofilm production in Staphylococcus aureus isolated from white coats of health university students

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Author(s):
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Batista, Isabela Rotta [1] ; Prates, Amanda Caroline Lima [1] ; Santos, Bruna de Souza [1] ; Araujo, Josimara Cristina Carvalho [1] ; Bonfim, Yan Christian de Oliveira [1] ; Pimenta Rodrigues, Marcus Vinicius [1] ; Morceli, Glilciane [1] ; Polettini, Jossimara [1] ; Cavalleri, Andressa Cortes [1] ; Winkelstroter, Lizziane Kretli [1] ; Pereira, Valeria Cataneli [1]
Total Authors: 11
Affiliation:
[1] Univ Oeste Paulista UNOESTE, Rua Jose Bongiovani, 700 Cidade Univ, BR-19050920 Presidente Prudente, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS; v. 18, n. 1 NOV 28 2019.
Web of Science Citations: 0
Abstract

This study aimed at detecting Staphylococcus aureus from white coats of college students and characterizing antimicrobial susceptibility and biofilm production. Bacterial samples (n = 300) were obtained from white coats of 100 college students from August 2015 to March 2017 S. aureus was isolated and it ` s resistance profile was assessed by antimicrobial disk-diffusion technique, screening for methicillin-resistant Staphylococcus aureus (MRSA), detection of mecA gene by PCR, and determination of staphylococcal cassette chromosome mec (SCCmec) by multiplex PCR. Congo red agar (CRA) and icaA and icaD genes by PCR were used for biofilm characterization. S. aureus was identified in 45.0% of samples. Resistance of S. aureus sample to antimicrobial was seen for penicillin (72.59%), erythromycin (51.85%), cefoxitin (20.74%), oxacillin (17.04%), clindamycin (14.81%) and levofloxacin (5.18%). MRSA was detected in 53.3% of the samples with SCCmec I (52.8%), SCCmec III (25%) and SCCmec IV (11.1%). Biofilm production was observed in 94.0% S. aureus samples. These data show that biosafety measures need to be enhanced in order to prevent dissemination of multiresistant and highly adhesive bacteria across other university settings, relatives, and close persons. (AU)

FAPESP's process: 16/15809-8 - Detection of the biofilm and oxacillin resistance in Staphylococcus aureus isolated from white coat
Grantee:Amanda Caroline Lima Prates
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 16/12252-2 - Determination of the antimicrobial sustetibility in Staphylococcus aureus from university coats
Grantee:Isabela Rotta Batista
Support Opportunities: Scholarships in Brazil - Scientific Initiation